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Volume 12, Number 5—May 2006
THEME ISSUE
Tuberculosis Special Section

Dispatch

Mycobacterium bovis Isolates with M. tuberculosis Specific Characteristics

Tanja Kubica*, Rimma Agzamova†, Abigail Wright‡, Galimzhan Rakishev†, Sabine Rüsch-Gerdes*, and Stefan Niemann*Comments to Author 
Author affiliations: *National Reference Center for Mycobacteria, Borstel, Germany; †National Center for Tuberculosis Problems, Almaty, Kazakhstan; ‡World Health Organization, Geneva, Switzerland

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Table 2

Genetic characteristics of type strains Mycobacterium tuberculosis H37 (ATCC 27294), M. bovis (ATCC19210), M. africanum (ATCC25420), and the strains analyzed*

Strains Test result
TbD1 RD1 RD3 RD4 RD5 RD9 RD10 RD12 IS1561 oxyR† gyrB‡
Kazakhstan (n = 8)§ 1 1 1 0 0 0 0 0 1 1 M. bovis
M. bovis ATCC 1 1 1 0 0 0 0 0 1 1 M. bovis
M. tuberculosis H37 0 1 1 1 1 1 1 1 1 0 M. tuberculosis
M. africanum ATCC 1 1 0 1 1 0 0 1 1 0 M. africanum

*RD, region of difference; 0, region deleted; 1, region present.
†Presence of oxyR mutation G to A at position 285; 1, polymorphism present; 0, polymorphism not present.
‡Classification according to gyrb-polymerase chain reaction restriction fragment length polymorphism analysis (Niemann et al. [3]).
§All 8 strains showed identical test results.

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