Volume 12, Number 5—May 2006
Dispatch
Heterogeneity among Mycobacterium ulcerans Isolates from Africa
Pieter Stragier*, Anthony Ablordey*, L. Manou Bayonne†, Yatta L. Lugor‡, Ireneaus S. Sindani§, Patrick Suykerbuyk*, Henry Wabinga¶, Wayne M. Meyers#, and Françoise Portaels*
Table 2
Primer sequence and location in Mycobacterium ulcerans and amplicon length at loci 1, 6, 9, and 33, resulting from a polymorphism in tandem repeat copy numbers
| Locus | Primer sequence |
Amplicon length |
|||||
|---|---|---|---|---|---|---|---|
| Forward primer (5´–3´) | Reverse primer (5´–3´) | Location | 1 copy | 2 copies | 3 copies | 4 copies | |
| 1 | GCTGGTTCATGCGTGGAAG | GCCCTCGGGAATGTGGTT | mu0115C04F | 380 | 433 | 486 | 539 |
| 6 | GACCGTCATGTCGTTCGATCCTAGT | GACATCGAAGAGGTGTGCCGTCT | mu0019B07G | 500 | 556 | – | – |
| 9 | GCCGAAGCCTTGTTGGACG | GGTTTCCCGCAGCATCTCG | mu0113D07F | 435 | 488 | – | – |
| 33 | CAAGACTCCCACCGACAGGC | CGGATCGGCACGGTTCA | mu0043E11R | 720 | 778 | 836 | – |
