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Volume 12, Number 6—June 2006

Dispatch

West Nile Virus Infection in Humans and Horses, Cuba

Maritza Pupo*Comments to Author , Maria Guadalupe Guzmán*, Roberto Fernández*, Alina Llop*, Félix Orlando Dickinson*, Daniel Pérez*, Raúl Cruz†, Tayri González†, Gonzalo Estévez†, Hiram González‡, Paulino Santos§, Gustavo Kourí*, Maya Andonova¶, Robbin Lindsay¶, Harvey Artsob¶, and Michael Drebot¶
Author affiliations: *Tropical Medicine Institute "Pedro Kouri," Havana, Cuba; †Ministry of Public Health, Havana, Cuba; ‡Instituto de Ecología y Sistemática, Havana, Cuba; §Instituto de Medicina Veterinaria, Havana, Cuba; ¶National Microbiology Laboratory, Winnipeg, Manitoba, Canada

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Table 1

Summary of horse sera tested for evidence of WNV infection by ELISA and PRNT*

Sample no. % inhibition (ELISA)
90% PRNT titer
Anti-WNV Antiflavivirus WNV SLEV Interpretation
C-7 65.3 65.2 160 20 WNV
C-13 58.9 60.4 320 WNV
C-19 63.2 63.3 320 WNV
C-20 74.6 62.2 640 WNV
C-2 44.4 60.4 20 SLEV
C-4 11.6 40.1 20 SLEV
C-5 67.5 67.6 20 SLEV
C-11 10.5 26.7 40 SLEV
C-12 32.2 68.0 20 SLEV
C-15 27.4 68.9 40 SLEV
C-16 64.6 65.7 40 SLEV
C-18 59.1 68.2 40 SLEV
C-10 62.7 66.3 80 40 Unidentified flavivirus
C-1 22.3 74.4 Unidentified flavivirus
C-3 40.5 54.1 Unidentified flavivirus
C-6 28.5 74.8 Unidentified flavivirus
C-9 40.9 73.6 Unidentified flavivirus
C-14 34.8 41.0 Unidentified flavivirus
C-21 9.9 68.4 Unidentified flavivirus

*WNV, West Nile virus; ELISA, enzyme-linked immunosorbent assay; PRNT, plaque reduction neutralization test; SLEV, Saint Louis encephalitis virus.

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