Emerging Infectious Disease ISSN: 1080-6059

Genetic Divergence of Toxoplasma gondii Strains Associated with Ocular Toxoplasmosis, Brazil

Asis Khan*, Catherine Jordan*, Cristina Muccioli†, Adriana L. Vallochi‡, Luiz V. Rizzo‡, Rubens Belfort†, Ricardo W.A. Vitor§, Claudio Silveira¶, and L. David Sibley*

Author affiliations: *Washington University School of Medicine, St. Louis, Missouri, USA; †Paulista School of Medicine, São Paulo, Brazil; ‡University of São Paulo, São Paulo, Brazil; §Federal University of Minas Gerais, Belo Horizonte, Brazil; ¶Clinica Silveira, Erechim, Brazil

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Figure 3

Neighbor-joining phylogram of 38 Toxoplasma gondii strains derived from polymerase chain reaction–restriction fragment length polymorphism typing at loci (SAG2, SAG3, GRA6, and BTUB). Distances were calculated according to Nei and Li (21) and the distance matrix analyzed using the phylogenetic analysis program PAUP*4.0b to generate an unrooted phylogram (22). The numbers on the branches indicate the bootstrap values (1,000 replicates). Strain designations are shown in the Table.

Figure 3. Neighbor-joining phylogram of 38 Toxoplasma gondii strains derived from polymerase chain reaction–restriction fragment length polymorphism typing at loci (SAG2, SAG3, GRA6, and BTUB). Distances were calculated according to Nei and Li (21) and the distance matrix analyzed using the phylogenetic analysis program PAUP*4.0b to generate an unrooted phylogram (22). The numbers on the branches indicate the bootstrap values (1,000 replicates). Strain designations are shown in the Table.

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