Genetic Divergence of Toxoplasma gondii Strains Associated with Ocular Toxoplasmosis, Brazil
Asis Khan*, Catherine Jordan*, Cristina Muccioli†, Adriana L. Vallochi‡, Luiz V. Rizzo‡, Rubens Belfort†, Ricardo W.A. Vitor§, Claudio Silveira¶, and L. David Sibley*
Author affiliations: *Washington University School of Medicine, St. Louis, Missouri, USA; †Paulista School of Medicine, São Paulo, Brazil; ‡University of São Paulo, São Paulo, Brazil; §Federal University of Minas Gerais, Belo Horizonte, Brazil; ¶Clinica Silveira, Erechim, Brazil
Figure 4. A phylogram of 35 Toxoplasma gondii strains was constructed from a Clustal alignment of UPRT-1 intron sequences using the phylogenetic analysis program PAUP*4.0b (22). The BioNeighbor-Joining algorithm was used to determine the divergence distance among different strains and generate an unrooted phylogram. Consensus trees were bootstrapped for 1,000 replicates and drawn with an arbitrary root according to the 50% majority rule. Strain designations are shown in the Table. A complete listing of intron sequences is found in Figure A1.
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