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Volume 12, Number 6—June 2006

Dispatch

Acanthamoeba Encephalitis in Patient with Systemic Lupus, India

Charudatt G. Shirwadkar*, Rohini Samant*, Milind Sankhe*, Ramesh Deshpande*, Shigeo Yagi†, Frederick L. Schuster†, Rama Sriram‡, and Govinda S. Visvesvara‡Comments to Author 
Author affiliations: *P.D. Hinduja National Hospital and Research Centre, Mumbai, India; †California Department of Health Services, Richmond, California, USA; ‡Centers for Disease Control and Prevention, Atlanta, Georgia, USA

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Figure 2

Results of polymerase chain reaction showing 230-bp and 161-bp bands for Balamuthia (lanes 1–6) and Acanthamoeba (lanes 7–12) mitochondrial 16S rRNA gene DNA. Lane 1, molecular mass marker; lane 2, absence of Balamuthia DNA in patient brain tissue (5-μL sample); lane 3, positive control (Balamuthia DNA); lane 4, negative control (Acanthamoeba DNA); lane 5, negative control (water); lane 6, absence of Balamuthia DNA in patient brain tissue (1-μL sample); lane 7, molecular mass marker; lane 8, Aca

Figure 2. Results of polymerase chain reaction showing 230-bp and 161-bp bands for Balamuthia (lanes 1–6) and Acanthamoeba (lanes 7–12) mitochondrial 16S rRNA gene DNA. Lane 1, molecular mass marker; lane 2, absence of Balamuthia DNA in patient brain tissue (5-μL sample); lane 3, positive control (Balamuthia DNA); lane 4, negative control (Acanthamoeba DNA); lane 5, negative control (water); lane 6, absence of Balamuthia DNA in patient brain tissue (1-μL sample); lane 7, molecular mass marker; lane 8, Acanthamoeba DNA isolated from patient brain tissue (5-μL sample); lane 9, absence of Balamuthia DNA isolated from amebae in patient sample; lane 10, positive control (Acanthamoeba DNA); lane 11, negative control (water); lane 12, Acanthamoeba DNA isolated from patient brain tissue (1-μL sample).

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