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Volume 12, Number 7—July 2006
Dispatch

Replicon Typing of Plasmids Encoding Resistance to Newer β-Lactams

Alessandra Carattoli*Comments to Author , Vivi Miriagou†, Alessia Bertini*, Alexandra Loli†, Celine Colinon‡, Laura Villa*, Jean M. Whichard§, and Gian Maria Rossolini‡
Author affiliations: *Istituto Superiore di Sanità, Rome, Italy; †Institute Pasteur, Athens, Greece; ‡Università di Siena, Siena, Italy; §Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Main Article

Figure

Figure. Restriction enzyme analysis of plasmids analyzed in this study. Numbers and letters above each lane indicate strain reference names as defined in the Table. M is the1-kb Plus DNA ladder (Gibco-BRL, Gaithersburg, MD, USA). Plasmids were transferred by conjugation or transformation in Escherichia coli K12 strains and purified by Midi-prep purification kit. Plasmids were digested with restriction endonuclease PstI and separated by agarose (1%) gel electrophoresis in 1× TBE buffer. DNA was stained with ethidium bromide and visualized under UV light. The replicons and the β-lactamase genes carried by each group of plasmids are indicated below the figure.

Main Article

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