Author affiliations: *Chiba Institute of Science, Choshi, Japan; †Azabu University, Sagamihara, Japan; ‡Shizuoka Saisei-kai General Hospital, Shizuoka, Japan; §National Institute of Infectious Diseases, Tokyo, Japan; ¶Tokyo Quarantine Station, Kawasaki, Japan; #The University of Tokyo, Tokyo, Japan
Figure 2. Pulsed-field gel electrophoresis analysis of NotI restriction fragment of Leptospira isolates from patient 2 and southern flying squirrels. Leptospira cells were lysed, and DNA was digested with restriction enzyme NotI in agarose gels. DNA in the gel was electrophoresed with 1% pulsed-field certified agarose in 0.5× Tris-borate-EDTA buffer under a pulse time of 10 s for 5 h and 30 s for 12 h, followed by 60 s for 7 h at 200 V. Lane 1, AM1; lane 2, AM2; lane 3, AM3; lane 4, AM7; lane 5, serovar Grippotyphosa strain Moskva V; lane 6, P10.2; lane 7, P10.1; lane 8, P5.4; λ phage DNA concatemer is used as a DNA size marker. Isolate AM8 showed an identical restriction fragment length polymorphism pattern to that of others.
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