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Volume 13, Number 12—December 2007

Dispatch

Viable Newcastle Disease Vaccine Strains in a Pharmaceutical Dump

Antonella Amendola*Comments to Author , Silvia Bianchi*, Marta Canuti*, Alessandra Zappa*, Giovanna Zanoni†, Raffaella Koncan†, Elisabetta Tanzi*, Giuseppe Cornaglia†, Alessandro Remo Zanetti*, and Giuseppe Tridente†
Author affiliations: *University of Milan, Milan, Italy; †University of Verona, Verona, Italy;

Main Article

Table

Comparison of Newcastle disease virus sequences

Classification* Amino acid sequence
Lentogenic 112(G/E)(R/K)Q(G/E)RL117
Velogenic 112(R/K)RQ(R/K)RF117
Strains† Nucleotide/amino acid sequence
ISM-1, ISM-2 GGG AGA CAG GGG CGC CTT
112G R Q G R L117
ISM-3, ISM-4 AGG AGA CAG AGA CGG TTT
112R R Q R R F117

*Amino acid sequences at the F protein cleavage site of lentogenic and velogenic strains of Newcastle disease virus.
†Nucleotide/amino acid sequences of the samples recovered from a dump at Istituto Sieroterapico Milanese (ISM), Milan, Italy, and analyzed in this study. ISM-1 and ISM-2 (GenBank accession nos. EU082818 and EU082819, respectively) were recovered in lyophilized form; ISM-3 and ISM-4 (GenBank accession nos. EU082820 and EU082821, respectively) were recovered in liquid form. The cleavage site sequences of ISM-1 and ISM-2 are typical of lentogenic strains; the cleavage site sequences of ISM-3 and ISM-4 are typical of velogenic strains.

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