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Volume 13, Number 3—March 2007

Research

In Vitro Cell Culture Infectivity Assay for Human Noroviruses

Timothy M. Straub*Comments to Author , Kerstin Höner zu Bentrup†, Patricia Orosz Coghlan‡, Alice Dohnalkova*, Brooke K. Mayer*1, Rachel A. Bartholomew*, Catherine O. Valdez*, Cynthia J. Bruckner-Lea*, Charles P. Gerba‡, Morteza A. Abbaszadegan§, and Cheryl A. Nickerson†1
Author affiliations: *Pacific Northwest National Laboratory, Richland, Washington, USA; †Tulane University School of Medicine, New Orleans, Louisiana, USA; ‡University of Arizona, Tucson, Arizona, USA; §Arizona State University, Tempe, Arizona, USA;

Main Article

Figure 2

Demonstration of cytopathic effect in infected tissue aggregates during the second infection trial. A) Uninfected aggregate, 24 h into the experiment. B) Cells infected with lysate from the first infection trial (P1) at 24 h postinfection. C) Stool sample flag2 at 24 s postinfection (P0). D) Stool sample 149 at 48 h postinfection (P0). Arrows indicate cells exhibiting unusual pathology.

Figure 2. Demonstration of cytopathic effect in infected tissue aggregates during the second infection trial. A) Uninfected aggregate, 24 h into the experiment. B) Cells infected with lysate from the first infection trial (P1) at 24 h postinfection. C) Stool sample flag2 at 24 s postinfection (P0). D) Stool sample 149 at 48 h postinfection (P0). Arrows indicate cells exhibiting unusual pathology.

Main Article

1Current affiliation: Arizona State University, Tempe, Arizona, USA

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