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Volume 13, Number 3—March 2007

Research

In Vitro Cell Culture Infectivity Assay for Human Noroviruses

Timothy M. Straub*Comments to Author , Kerstin Höner zu Bentrup†, Patricia Orosz Coghlan‡, Alice Dohnalkova*, Brooke K. Mayer*1, Rachel A. Bartholomew*, Catherine O. Valdez*, Cynthia J. Bruckner-Lea*, Charles P. Gerba‡, Morteza A. Abbaszadegan§, and Cheryl A. Nickerson†1
Author affiliations: *Pacific Northwest National Laboratory, Richland, Washington, USA; †Tulane University School of Medicine, New Orleans, Louisiana, USA; ‡University of Arizona, Tucson, Arizona, USA; §Arizona State University, Tempe, Arizona, USA;

Main Article

Table 3

Relative increases in viral RNA as measured by limiting dilution PCR*

Effective dilution of working viral stock applied to cellsHours postinfection
1246672
1:10
+
+
+
ND
1:100
+
+
+
ND
1:1,000
-
-
+
+
Negative control----

*ND, not done.

Main Article

1Current affiliation: Arizona State University, Tempe, Arizona, USA

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