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Volume 13, Number 3—March 2007

Dispatch

Rapidly Fatal Acanthamoeba Encephalitis and Treatment of Cryoglobulinemia

Wouter Meersseman*Comments to Author , Katrien Lagrou*, Raf Sciot*, Johan de Jonckheere†, Christine Haberler‡, Julia Walochnik‡, Willy E. Peetermans*, and Eric van Wijngaerden*
Author affiliations: *Gasthuisberg University Hospital, Leuven, Belgium; †Scientific Institute of Public Health, Brussels, Belgium; ‡Medical University of Vienna, Vienna, Austria;

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Figure 2

A) Cysts in a vessel wall (arrows) of the patient (hematoxylin and eosin stain, magnification ×250). Inset shows a cyst at higher magnification (hematoxylin and eosin stain, magnification ×800). B) Immunohistochemical staining with antibody to Acanthamoeba cysts within vessel walls (magnification ×250). C) Polyacrylamide gel electrophoresis of PCR products for Acanthamoeba spp., Naegleria spp., and Balamuthia mandrillaris using JDP primers for a diagnostic small subunit rDNA fragment. M, molecul

Figure 2. A) Cysts in a vessel wall (arrows) of the patient (hematoxylin and eosin stain, magnification ×250). Inset shows a cyst at higher magnification (hematoxylin and eosin stain, magnification ×800). B) Immunohistochemical staining with antibody to Acanthamoeba cysts within vessel walls (magnification ×250). C) Polyacrylamide gel electrophoresis of PCR products for Acanthamoeba spp., Naegleria spp., and Balamuthia mandrillaris using JDP primers for a diagnostic small subunit rDNA fragment. M, molecular mass marker; Pat, patient; Neg, negative control; Pos, positive control.

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