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Volume 13, Number 3—March 2007

Research

Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1

Stephen Mark Tompkins*1Comments to Author , Zi-Shan Zhao*, Chia-Yun Lo*, Julia A. Misplon*, Teresa Liu*, Zhiping Ye*, Robert J. Hogan†, Zhengqi Wu*, Kimberly A. Benton*, Terrence M. Tumpey‡, and Suzanne L. Epstein*
Author affiliations: *Food and Drug Administration, Bethesda, Maryland, USA; †University of Georgia, Athens, Georgia, USA; ‡Centers for Disease Control and Prevention, Atlanta, Georgia, USA;

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Figure 3

Results of matrix protein 2 (M2)–DNA vaccination, showing protection against divergent influenza viruses. Mice (8–10 per group) were vaccinated with DNA as described in Methods except at a dose of 100 μg/mouse. Approximately 2 weeks after the last dose of DNA, mice were challenged with 7× the 50% lethal dose (LD50) of virus and monitored for survival. A) A/PR/8 challenge: Cumulative survival rate of mice vaccinated with M-DNA or M2-DNA was significantly higher than that of mice vaccinated with B

Figure 3. Results of matrix protein 2 (M2)–DNA vaccination, showing protection against divergent influenza viruses. Mice (8–10 per group) were vaccinated with DNA as described in Methods except at a dose of 100 μg/mouse. Approximately 2 weeks after the last dose of DNA, mice were challenged with 7× the 50% lethal dose (LD50) of virus and monitored for survival. A) A/PR/8 challenge: Cumulative survival rate of mice vaccinated with M-DNA or M2-DNA was significantly higher than that of mice vaccinated with B/NP-DNA (p<0.001, log rank). B) A/FM challenge: Cumulative survival rate differed significantly among groups (p = 0.041, log-rank), although in post hoc Holm-Sidak tests, pairs did not differ significantly (p≥0.05).

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1Current affiliation: University of Georgia, Athens, Georgia, USA

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