Volume 13, Number 5—May 2007
Chikungunya Virus in US Travelers Returning from India, 2006
|Primer||Genome position*||Sequence (5′→3′)||Sensitivity†||Specificity‡|
*On the basis of CHIKV prototype strain S27, GenBank accession no. NC_004162.
†Absolute no. of PFU detected in triplicate testing.
‡No reactivity was observed with the following viruses: o’nyong nyong, Ross River, Mayaro, Semliki Forest, Sindbis, western
equine encephalitis, eastern equine encephalitis, and Venezuelan equine encephalitis subtypes 1AB, 1C, 1D, and 1E.
§Primer labeled at the 5′ terminus with 5-FAM and 3′ Black Hole Quencher 1 (Operon Biotechnologies Inc., Huntsville, AL, USA).
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