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Volume 13, Number 6—June 2007

Research

Minority-Variant pfcrt K76T Mutations and Chloroquine Resistance, Malawi

Jonathan J. Juliano*, Jesse J. Kwiek*, Kathryn Cappell*, Victor Mwapasa†, and Steven R Meshnick*Comments to Author 
Author affiliations: *University of North Carolina, Chapel Hill, North Carolina, USA; †University of Malawi, Blantyre, Malawi

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Figure

Evaluation of sensitivity of multiple site–specific heteroduplex tracking assay (MSS-HTA). A) MSS-HTA tested against known concentrations of Plasmodium falciparum DNA. Visible bands representing mutant DNA remain until the 1% population (lanes N and O). B) the same dilution series assayed with allele-restricted PCR (ARPCR), where visible mutant bands (366 bp) are not seen past the 20% mutant population (lanes I and J). The lanes marked with W and M represent wild-type and mutant restricted react

Figure. Evaluation of sensitivity of multiple site–specific heteroduplex tracking assay (MSS-HTA). A) MSS-HTA tested against known concentrations of Plasmodium falciparum DNA. Visible bands representing mutant DNA remain until the 1% population (lanes N and O). B) the same dilution series assayed with allele-restricted PCR (ARPCR), where visible mutant bands (366 bp) are not seen past the 20% mutant population (lanes I and J). The lanes marked with W and M represent wild-type and mutant restricted reactions, respectively. HTA, heteroduplex tracking assay; *, base pair ladder; P, probe alone.

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