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Volume 13, Number 7—July 2007

Dispatch

Rocky Mountain Spotted Fever, Colombia

Marylin Hidalgo*, Leonora Orejuela†, Patricia Fuya*, Pilar Carrillo‡, Jorge Hernandez§, Edgar Parra*, Colette Keng¶, Melissa Small¶, Juan P. Olano¶, Donald Bouyer¶, Elizabeth Castaneda*, David H. Walker¶, and Gustavo Valbuena†¶Comments to Author 
Author affiliations: *Instituto Nacional de Salud, Bogota, Colombia; †School of Medicine at Universidad de Los Andes, Bogota, Colombia; ‡Secretaria de Salud de Cundinamarca, Colombia; §Hospital Salazar, Villeta, Cundinamarca, Colombia; ¶University of Texas Medical Branch, Galveston, Texas, USA;

Main Article

Table 1

Rocky Mountain spotted fever patients and findings, Colombia, 2003–2004

Patient
Confirmatory methods
Adult ticks collected in area where patients lived
PCR
Sequence
IHA*
Animal inoculation
Culture
1
Genes
Primers
Homology with Rickettsia rickettsii
Positive with rabbit anti–spotted fever group rickettsial antibody
Not done
Not done
15 Amblyomma cajennense, 184 Rhipicephalus sanguineus, 7 Anocentor nitens, and 8 Amblyomma spp.
17-kDa
17kD1/2
100% (Sheila Smith)
2Genes
Primers
Homology with 
R. rickettsii
Positive with rabbit anti–spotted fever group rickettsial antibody24 h and 48 h after fever onset, 2 guinea pigs were euthanized for culture and PCR analysis of spleensVero cells with cytopathic changes after 1 week36 A. cajennense, 13 R. sanguineus, and 38 Boophilus microplus
gltACS78/32399% (Bitterroot and others)
gltACS5/694% (Bitterroot)
OmpA190.70/70198% (strain 1995HO2 and others)
OmpBrOmpB.20-278898% (GenBank accession no. X16353.1)

*Immunohistochemical analysis.

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