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Volume 13, Number 7—July 2007

Dispatch

Blood Screening for Influenza

Michael Kai Hourfar*, Anna Themann*, Markus Eickmann†, Pilaipan Puthavathana‡, Thomas Laue§, Erhard Seifried*, and Michael Schmidt*Comments to Author 
Author affiliations: *German Red Cross, Frankfurt, Germany; †Phillips University, Marburg, Germany; ‡Mahidol University, Bangkok, Thailand; §QIAGEN, Hamburg, Germany;

Main Article

Table 2

Analytical sensitivity for avian influenza (H5N1) virus subtype for plasma samples*

PFU (H5N1) spiked 
in minipoolsNo. positive/
no. tested% Positive
125.08/8100
62.58/8100
31.38/8100
15.68/8100
7.87/887.5
3.97/887.5
1.94/850
0.93/837.5
0.00/80

*Influenza (H5N1) standard was extracted from 9.6 mL of 96 pooled donor samples after centrifugation. Five microliters of 75-µL nucleic acid extract was analyzed. The 95% detection limit was 7.4 PFU/mL; the 50% detection limit was 2.5 PFU/mL.

Main Article

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