TY - JOUR AU - Gerrish, Robert S. AU - Lee, James E. AU - Reed, June AU - Williams, Joel AU - Farrell, Larry D. AU - Spiegel, Kathleen M. AU - Sheridan, Peter P. AU - Shields, Malcolm S. T1 - PCR versus Hybridization for Detecting Virulence Genes of Enterohemorrhagic Escherichia coli T2 - Emerging Infectious Disease journal PY - 2007 VL - 13 IS - 8 SP - 1253 SN - 1080-6059 AB - We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. Both methods showed 100% of the chromosomal and phage genes: eae, stx, and stx2. PCR did not detect 4%–20% of hybridizable plasmid genes: hlyA, katP, espP, toxB, open reading frame (ORF) 1, and ORF2. KW - Stx1 KW - Stx2 KW - eae KW - ehlx KW - HlyA KW - ToxB KW - ORF1 KW - ORF2 KW - KatP KW - EspP KW - bfp KW - EAF KW - pOSAK1 KW - pB171 KW - pO157 KW - EHEC KW - virulence factors KW - dispatch KW - United States DO - 10.3201/eid1308.060428 UR - https://wwwnc.cdc.gov/eid/article/13/8/06-0428_article ER - End of Reference