TY - JOUR AU - Li, Wenjun AU - Raoult, Didier AU - Fournier, Pierre-Edouard T1 - Genetic Diversity of Bartonella henselae in Human Infection Detected with Multispacer Typing T2 - Emerging Infectious Disease journal PY - 2007 VL - 13 IS - 8 SP - 1178 SN - 1080-6059 AB - We applied multispacer typing (MST) by incorporating 9 variable intergenic spacers to Bartonella henselae DNA detected in lymph node biopsy specimens from 70 patients with cat-scratch disease (CSD), in cardiac valve specimens from 2 patients with endocarditis, and in 3 human isolates from patients with bacillary angiomatosis, CSD, and endocarditis. Sixteen MST genotypes were found, 5 previously identified in cats and 11 new. Of the studied DNA, 78.7% belonged to 2 genotypes, which were phylogenetically organized into 4 lineages. Human strains were mostly grouped within 2 lineages, previously identified as Marseille and Houston-1. Our results suggest a greater genetic diversity in human-infecting B. henselae than what has previously been evaluated by using other genotyping methods. However, the diversity is not significantly different from that of cat strains. MST is thus a suitable genotyping tool for evaluating the genetic heterogeneity of B. henselae among isolates obtained from human patients. KW - Bartonella KW - genetic heterogeneity KW - gene sequences KW - multispacer typing KW - genotyping KW - research KW - France DO - 10.3201/eid1308.070085 UR - https://wwwnc.cdc.gov/eid/article/13/8/07-0085_article ER - End of Reference