Protochlamydia naegleriophila as Etiologic Agent of Pneumonia
Figure 2. A) Intra and inter-run reproducibility of the real-time PCR assessed on duplicate of plasmidic positive controls performed at 10-fold dilutions from 105 to 101 plasmid/μL during 6 successive runs. Standard deviations show the intra-run reproducibility of the real-time PCR. B) Plots of the cycle threshold (Ct) of first and second duplicates, showing intra-run and inter-run variability of the real-time PCR between duplicates of positive control. 95% confidence interval is shown by the dashed lines. C) Bland-Altman graph showing the difference of Ct of both duplicates according to the mean of the Ct of duplicates. The dashed line shows the 95% confidence interval (i.e., limit of agreement).