Volume 14, Number 10—October 2008
Serogroup A Neisseria meningitidis with Reduced Susceptibility to Ciprofloxacin
To the Editor: Reduced susceptibility to ciprofloxacin of Neisseria meningitidis has been reported with increasing frequency since 1992, mainly because of mutations in the quinolone resistance determining regions (QRDRs) of the gyrase and topoisomerase IV genes (1,2). Reduced fluoroquinolone susceptibility due to gyrase A mutations in serogroup A strains has previously been reported from a 2005 outbreak in Delhi, India (1). We describe 2 clinical isolates of serogroup A N. meningitidis with reduced ciprofloxacin susceptibility that were recognized in March 2003 and April 2006 in Israel, a country with low incidence of invasive meningococcal disease (<2/100,000/laboratory-confirmed cases/year) in which this serogroup accounts for <2% of cases (data from the National Center for Meningococci, Tel Hashomer, Israel).
The 2 isolates in question (M12/03 and M24/06; suffixes denote year of isolation) were compared with 2 fully susceptible strains, M44/01 and M23/00 (Appendix Table [PDF - 24 KB - 1 page]). MICs were measured by Etest (AB Biodisk, Solna, Sweden) on Mueller-Hinton agar (Difco Laboratories, Detroit, MI, USA) supplemented with 5% sheep blood. Demographic information was obtained from the Israel Ministry of Health Department of Epidemiology.
Chromosomal DNA was isolated by using the NucleoBond kit (Macherey-Nagel, Düren, Germany). The location of the QRDR in gyrase and topoisomerase IV genes was based upon prior studies in meningococci (Appendix Table [PDF - 24 KB - 1 page]) and on the complete sequence of strain N. meningitidis Z2491 (serogroup A; GenBank accession no. NC_003116). We amplified and sequenced extended regions encompassing the QRDRs by using the upstream and downstream primer pairs in gyrA (522 bases) 5′-GTTCCGCGTCAAAATATGCT-3′, 5′-CCGAAATTGACGGTTTCTTC-3′; gyrB (649 bases) 5′-GGTTTGACCTGCGTGTTGTC-3′, 5′-CGGCTGGGCGATATAGATG-3′; parC (635 bases) 5′-CACTATGGTTTGCCGTTTTG-3′, 5′-GATTTCGGACAACAGCAATTC-3′; and parE (610 bases) 5′-GGACAGGATGGCGATTTTG-3′, 5′-CGTCAGCAACTTCATCAACC-3′. PCR was performed by using Taq DNA polymerase (New England BioLabs, Beverly, MA, USA). DNA sequencing was performed using the ABI PRISM 3700 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). Screening for plasmid-mediated quinolone resistance genes was carried out by multiplex PCR amplification of qnrA, qnrB, and qnrS as previously described (3). Multilocus sequence typing (MLST) was carried out by using the primers, protocols, and databases available from the Neisseria MLST website (http://pubmlst.org/neisseria) (4).
The Appendix Table [PDF - 24 KB - 1 page] shows our results and condenses previously published findings. The ciprofloxacin MIC for M24/06 was 42- to 125-fold higher than for susceptible strains and consistently 2-fold higher than that for M12/03. We have not referred to our isolates as resistant, because M12/03 would be categorized as “intermediate” by Clinical Laboratory Standards Institute breakpoints (5). The extended QRDRs in gyrA and parC of M44/01 (susceptible) were identical to those of N. meningitidis Z2491. M24/06 and M12/03 had a Thr91Ile mutation in gyrA. M24/06 also had Asn103Asp, Ile111Val, and Val120Ile mutations in gyrA (Appendix Table [PDF - 24 KB - 1 page]; 1). In M12/03, an Ala78Val mutation was found in gyrA, and new mutations Ile474Leu and Thr365Ala were found in gyrB and parE, respectively. No parC mutations were found.
Previous reports identified chromosomal mutations in N. meningitidis (Appendix Table [PDF - 24 KB - 1 page]). M24/06 and M12/03 possess the same Thr91Ile mutation in gyrA as a 2002 serogroup B isolate from Spain (Appendix Table [PDF - 24 KB - 1 page]) that had a similar increase in ciprofloxacin MIC (0.12 mg/L). The Thr91Ile mutation is homologous with the Ser83Leu mutation in gyrA of Escherichia coli that is responsible for a 60-fold increase in ciprofloxacin MICs (6). Further mutations in a primary target enzyme (gyrase) have been associated with additional 2-fold increases in the MIC of ciprofloxacin (7). The level of resistance observed in M24/06 might suggest additional mechanisms. An efflux pump mechanism is unlikely; we showed no reduction in MICs in the presence of reserpine (Appendix Table [PDF - 24 KB - 1 page]) and this organism was fully susceptible to penicillin, tetracycline, erythromycin, and Triton X-100 (data not shown). This finding suggests the absence of an efflux pump encoded by a mutated mtrRCDE (8). Neither M24/06 nor M12/03 had plasmid-mediated genes qnr genes or elevated kanamycin MICs, suggesting the presence of aac(6′)-Ib-cr. Both of these genes can confer low-level quinolone-resistance and facilitate the emergence of higher level resistance (9) (data not shown).
MLST showed that M24/06 and M12/03 did not derive from a single clone after selection of the T91I mutation. M12/03 was sequence type (ST) 2 and was isolated from a recent immigrant from Russia, which is the origin of most ST 2 strains deposited in the Neisseria MLST database (29/34 records; 85%). M24/06 was ST4789 in the ST5 clonal complex, isolated from a person who had immigrated many years previously from Romania. ST4789 has been encountered only once previously, in Dhaka, Bangladesh.
Disease associated with serogroup A N. meningitidis has been extremely unusual in Israel (10) and has remained rare. This serogroup comprised only 9 (1.9%) of all 463 isolates submitted during 1997–2006 (data from the National Center for Meningococci).
The isolates described in our study confirm that serogroup A should be added to the list of meningococci with the potential for reduced fluoroquinolone susceptibility and raise the question why they have appeared in a region with particularly low serogroup A meningococcal disease incidence while frequently encountered serogroups have remained fully susceptible. The importance of continuous monitoring for reduced ciprofloxacin susceptibility in these more prevalent serogroups has been emphasized by the recent replacement of rifampin by ciprofloxacin as the preferred agent for chemoprophylaxis of meningococcal disease in adults in Israel.
- Singhal S, Purnapatre KP, Kalia V, Dube S, Nair D, Deb M, Ciprofloxacin-resistant Neisseria meningitidis, Delhi, India. Emerg Infect Dis. 2007;13:1614–6.
- Centers for Diseases Control and Prevention. Emergence of fluoroquinolone-resistant Neisseria meningitidis—Minnesota and North Dakota, 2007–2008. MMWR Morb Mortal Wkly Rep. 2008;57:173–5.
- Robicsek A, Strahilevitz J, Sahm DF, Jacoby GA, Hooper DC. qnr Prevalence in ceftazidime-resistant Enterobacteriaceae isolates from the United States. Antimicrob Agents Chemother. 2006;50:2872–4.
- Jolley KA, Chan MS, Maiden MC. mlstdbNet–distributed multi-locus sequence typing (MLST) databases. BMC Bioinformatics. 2004;5:86.
- Clinical Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing, 18th informational supplement. CLSI document M100-S18. Wayne (PA): The Institute; 2008.
- Hooper DC, Rubinstein E. Mechanisms of quinolone resistance. Quinolone antimicrobial agents. 3rd ed. Washington: American Society for Microbiology Press; 2003. p. 41.
- Bagel S, Hullen V, Wiedemann B, Heisig P. Impact of gyrA and parC mutations on quinolone resistance, doubling time, and supercoiling degree of Escherichia coli. Antimicrob Agents Chemother. 1999;43:868.
- Shafer WM, Veal WL, Lee EH, Zarantonelli L, Balthazar JT, Rouquette C. Genetic organization and regulation of antimicrobial efflux systems possessed by Neisseria gonorrhoeae and Neisseria meningitidis. J Mol Microbiol Biotechnol. 2001;3:219–24.
- Robicsek A, Strahilevitz J, Jacoby GA, Macielag M, Abbanat D, Hye PC, Fluoroquinolone-modifying enzyme: a new adaptation of a common aminoglycoside acetyltransferase. Nat Med. 2006;12:83.
- Block C, Roitman M, Bogokowsky B, Meizlin S, Slater PE. Forty years of meningococcal disease in Israel: 1951–1990. Clin Infect Dis. 1993;17:126–32.
Suggested citation for this article: Strahilevitz J, Adler A, Smollan G, Temper V, Keller N, Block C. Serogroup A Neisseria meningitidis with reduced susceptibility to ciprofloxacin [letter]. Emerg Infect Dis [serial on the Internet]. 2008 Oct [date cited]. Available from http://wwwnc.cdc.gov/eid/article/14/10/08-0252
- Page created: July 13, 2010
- Page last updated: July 13, 2010
- Page last reviewed: July 13, 2010
- Centers for Disease Control and Prevention,
National Center for Emerging and Zoonotic Infectious Diseases (NCEZID)
Office of the Director (OD)