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Volume 14, Number 12—December 2008

Dispatch

Multiple Francisella tularensis Subspecies and Clades, Tularemia Outbreak, Utah

Jeannine M. Petersen, Jennifer K. Carlson, Gabrielle Dietrich, Rebecca J. Eisen, Jana Coombs, Aimee M. Janusz, JoDee Summers, Charles Ben Beard, and Paul S. MeadComments to Author 
Author affiliations: Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (J.M. Petersen, J.K. Carlson, G. Dietrich, R.J. Eisen, A.M. Janusz, C.B. Beard, P.S. Mead); Utah Department of Health, Salt Lake City, Utah, USA (J. Coombs, J. Summers)

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Figure 1

Dendrogram based on PmeI pulsed-field gel electrophoresis (PFGE) patterns of Francisella tularensis type A isolates. The dendrogram was constructed by using Dice similarity coefficients (1.5% optimization and 1.5% tolerance) and unweighted pair group method with averages. Strains WY96–3418, ATCC 6223, SCHU S4, and MA00–2972 were included as known A1 and A2 controls for creation of the dendrogram. Control strains were previously identified as either A1 or A2 by multiple methods including multilocus variable number tandem repeat analysis, PFGE, housekeeping gene sequence analysis, whole genome sequencing, and Indel analysis (1,2,4–7,9).

Figure 1. Dendrogram based on PmeI pulsed-field gel electrophoresis (PFGE) patterns of Francisella tularensis type A isolates. The dendrogram was constructed by using Dice similarity coefficients (1.5% optimization and 1.5% tolerance) and unweighted pair group method with averages. Strains WY96–3418, ATCC 6223, SCHU S4, and MA00–2972 were included as known A1 and A2 controls for creation of the dendrogram. Control strains were previously identified as either A1 or A2 by multiple methods including multilocus variable number tandem repeat analysis, PFGE, housekeeping gene sequence analysis, whole genome sequencing, and Indel analysis (1,2,47,9).

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