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Volume 14, Number 12—December 2008

Letter

Human Case of Rickettsia felis Infection, Taiwan

Kun-Hsien Tsai, Hsiu-Ying Lu, Jih-Jin Tsai, Sheng-Kai Yu, Jyh-Hsiung Huang, and Pei-Yun ShuComments to Author 
Author affiliations: Centers for Disease Control, Taipei, Taiwan, Republic of China (K.-H. Tsai, H.-Y. Lu, S.-K. Yu, J.-H. Huang, P.-Y. Shu); Kaohsiung Medical University, Kaohsiung, Taiwan, Republic of China (J.-J. Tsai)

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Table

Primer sequences for the SYBR-based real-time PCR assay*

Primer Genomic region Sequence (5′ → 3′) Amplicon size, bp
RR-F8 17-kDa (Rickettsia spp.) GGC GGY GCA TTA CTT GGT TCT CAA TTC GC 304
RR-R12 GTT TTC CSC CTA TTA CAA CTG TTT GAG T
RR-F1 groEL gene (Rickettsia spp.) AAA ATG GTT GCT GAG CTT GAA AAT CCT TT 191
RR-R2 ACT TTC AAA CCA CCA CGT AAT CTA TTG AC
RR-F22 ompB gene (Rickettsia spp.) ATG GTR TAT GGG CWA AAC CTT TCT ATA 330
RR-R25 TAG MMT CGA AGA AGT AAC GCT GAC TTT
RST-14F 56-kDa gene (Orientia tsutsugamushi) CCA TTT GGT GGT ACA TTA GCT GCA GGT 233
RST-6R TCA CGA TCA GCT ATA CTT ATA GGC A
OMP3 com-1 (Coxiella burnetii) GAA GCG CAA CAA GAA GAA CAC 438
OMP4 TTG GAA GTT ATC ACG CAG TTG

*groEL, 60-kDa heat-shock protein; ompB, outer membrane protein B; com-1, 27-kDa outer membrane protein.

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