Human Illnesses Caused by Opisthorchis felineus Flukes, Italy
Figure 2. Alignment of the internal transcribed spacer 2 region of rDNA. Patient 1, DNA from eggs of a patient of the August 2007 outbreak; patient 2, DNA from eggs of the patient of the October–November 2007 outbreak; metacercaria collected from a tench (Tinca tinca) from Lake Bolsena (Latium region, central Italy); Opisthorchis felineus, reference DNA from an adult worm of O. felineus from Germany; O. viverrini, reference DNA from an adult worm of O. viverrini from Thailand; C. sinensis, DNA from an adult worm of Clonorchis sinensis from China. Primer sequences are underlined, different bases are in boldface, and gaps are represented by dashes.