Volume 14, Number 12—December 2008
Dispatch
Transmission of Atypical Bovine Prions to Mice Transgenic for Human Prion Protein
Vincent Béringue
, Laëtitia Herzog, Fabienne Reine, Annick Le Dur, Cristina Casalone, Jean-Luc Vilotte, and Hubert Laude
, Laëtitia Herzog, Fabienne Reine, Annick Le Dur, Cristina Casalone, Jean-Luc Vilotte, and Hubert LaudeTable
Transmission of classical and atypical BSE isolates to transgenic mice expressing human prion protein (Met129)*
| Isolate | Origin (identification no.) | 1st passage |
2nd passage |
3rd passage |
|||||
|---|---|---|---|---|---|---|---|---|---|
| Total no. affected† | Mean survival time‡ | Total no. affected† | Mean survival time‡ | Total no. affected† | Mean survival time‡ | ||||
| BSE | France (3) | 1/6§ | 872 | 6/7 | 568 ± 65 | 8/8 | 523 ± 22 | ||
| France (3) | 2/6 | 627, 842 | |||||||
| Germany¶ | 1/4§ | 802 | 6/6 | 677 ± 54 | 8/8 | 555 ± 24 | |||
| Italy (128204) | 0/5 | 606–775 | |||||||
| Belgium | 0/4 | 696–829 | |||||||
| L-type | Italy (1088) | 9/9 | 607 ± 23 | 11/11 | 653 ± 13 | NA | |||
| France (7) | 7/7 | 574 ± 35 | 0/8# | >450 | |||||
| France (10) | 8/8 | 703 ± 19 | |||||||
| France (11) | 9/9 | 647 ± 26 | |||||||
| H-type | France (1) | 0/6 | 376–721 | 0/7 | 350–850 | NA | |||
| France (2) | 0/6 | 313–626 | 0/8 | 302–755 | NA | ||||
| France (5) | 0/10 | 355–838 | NA | ||||||
*BSE, bovine spongiform encephalopathy; NA, not available (experiments still ongoing).
†Mice with neurologic signs and positive for protease-resistant prion protein by Western blotting.
‡Days ± SE of the mean. For mice with negative results, only the range of survival time is given.
§First passage performed on hemizygous mice. Note that the primary transmission of France (3) isolate was performed on both hemizygous and homozygous mice.
¶One passage on transgenic mice expressing bovine prion protein (7).
#Ongoing experiment.
