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Volume 14, Number 12—December 2008

Dispatch

Novel Borna Virus in Psittacine Birds with Proventricular Dilatation Disease

Kirsi S. Honkavuori, H.L. Shivaprasad, Brent L. Williams, Phenix-Lan Quan, Mady Hornig, Craig Street, Gustavo Palacios, Stephen K. Hutchison, Monique Franca, Michael Egholm, Thomas BrieseComments to Author , and W. Ian Lipkin
Author affiliations: Columbia University, New York, NY, USA (K.S. Honkavuori, B.L. Williams, P.-L. Quan, M. Hornig, C. Street, G. Palacios, T. Briese, W.I. Lipkin); University of California Animal Health and Food Safety Laboratory System–Fresno Branch, Davis, California, USA (H.L. Shivaprasad, M. Franca); 454 Life Sciences, Branford, Connecticut, USA (S.K. Hutchison, M. Egholm)

Main Article

Table 1

Real-time PCR measurement of viral sequences in birds with PPD*

Case no. Species† Age/sex PDD/ control Primer/probe set Organ Ct Virus copies‡
(300 ng total RNA)
1034 Canindae macaw 30 y/M PDD 1034–1322 Brain 17.94 8.88 × 107
Adrenal gland 20.51 8.77 × 106





Proventriculus and gizzard
25.26
1.22 × 105
1322 Vinaceous Amazon 1 y/M PDD 1034–1322 Brain 17.64 1.17 × 108





Proventriculus
28.47
6.76 × 103
1367 Canindae macaw 30 y/F PDD 1367 Brain 27.33 8.89 × 103
Adrenal gland 18.92 3.87 × 106





Proventriculus
23.82
1.13 × 105
5473
Leadbetter’s cockatoo
17 y/M
Control
1034–1322 and 1367
Brain
>36§
Negative
4858
Long-tailed parakeet
Adult/M
Control
1034–1322 and 1367
Brain
>36§
Negative
2020
Cockatiel
Young/M
Control
1034–1322 and 1367
Brain
>36§
Negative
3616 Eclectus parrot 0.25 y/F Control 1034–1322 and 1367 Brain >36§ Negative

*PPD, poventricular dilatation disease; Ct, cycle threshold.
†All birds were captive animals from California, USA.
‡Copy numbers were calculated on the basis of a standard curve generated from cloned target sequences.
§A Ct of >36 was rated as negative based on the highest dilution of standard representing 5 copies.

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