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Volume 14, Number 2—February 2008
Research

Genetic Determinants of Virulence in Pathogenic Lineage 2 West Nile Virus Strains

Elizabeth M. Botha*, Wanda Markotter*, Mariaan Wolfaardt*, Janusz T. Paweska†, Robert Swanepoel†, Gustavio Palacios‡, Louis H. Nel*, and Marietjie Venter*Comments to Author 
Author affiliations: *University of Pretoria, Pretoria, South Africa; †National Institute for Communicable Diseases, Sandringham, South Africa; ‡Columbia University, New York, New York, USA;

Main Article

Figure 2

A) Amino acid differences between South African lineage 2 strains of West Nile virus (WNV) strains sequenced in the present study and previously published lineage 2 strains. Strain SA381/00 is less neuroinvasive than the highly neuroinvasive strains SA93/01, H442, and SPU116/89. Light gray, hydrophobic amino acids; dark gray, hydrophilic amino acids; black, structural-determining amino acids; white blocks, deletion of the glycosylation site in the envelope protein of the B956D117B3 strain. Numbering is according to the SA381/00 genome position for specific genes. Cap, capsid; prM, premembrane; NS, nonstructural. B) Nucleotide differences in the noncoding 5′ and 3′ regions of lineage 2 strains. Numbering is according to WNV strain SA381/00. Black, deletions; gray, nucleotide differences. The length of each genome is given in the last column. (Strain AnMg798 is incomplete in the GenBank database and may thus be longer than indicated.)

Figure 2. A) Amino acid differences between South African lineage 2 strains of West Nile virus (WNV) strains sequenced in the present study and previously published lineage 2 strains. Strain SA381/00 is less neuroinvasive than the highly neuroinvasive strains SA93/01, H442, and SPU116/89. Light gray, hydrophobic amino acids; dark gray, hydrophilic amino acids; black, structural-determining amino acids; white blocks, deletion of the glycosylation site in the envelope protein of the B956D117B3 strain. Numbering is according to the SA381/00 genome position for specific genes. Cap, capsid; prM, premembrane; NS, nonstructural. B) Nucleotide differences in the noncoding 5′ and 3′ regions of lineage 2 strains. Numbering is according to WNV strain SA381/00. Black, deletions; gray, nucleotide differences. The length of each genome is given in the last column. (Strain AnMg798 is incomplete in the GenBank database and may thus be longer than indicated.)

Main Article

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