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Volume 14, Number 2—February 2008

Research

Genetic Characterization of Feline Leukemia Virus from Florida Panthers

Meredith A. Brown*, Mark W. Cunningham†, Alfred L. Roca*‡§, Jennifer L. Troyer*§, Warren E. Johnson*, and Stephen J. O’Brien*Comments to Author 
Author affiliations: *National Cancer Institute, Frederick, Maryland, USA; †Florida Fish and Wildlife Conservation Commission, Gainesville, Florida, USA; ‡University of Illinois at Urbana-Champaign, Urbana, Illinois, USA; §SAIC-Frederick, Frederick, Maryland, USA;

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Figure 4

Phylogenetic trees of panther feline leukemia virus (FeLV-Pco) and domestic cat FeLV nucleotide sequences. A) Midpoint rooted maximum-likelihood phylogram based on 1,698 bp of env sequences. B) Midpoint rooted maximum-likelihood phylogram based on 463 bp of 3’ long terminal repeat (LTR) sequences. Consensus FeLV-Pco sequences of clones generated from 5 env and 4 LTR panthers and reference domestic cat sequences are shown. The number of FeLV-Pco–cloned PCR products used in each consensus sequence is indicated in parentheses. The arrow indicates the monophyletic clade of all FeLV-Pco sequences. A similar topology, including the monophyletic clade, was obtained by using the different FeLV-Pco clone sequences rather than a consensus. The year of panther sampling is indicated as a suffix, e.g., Pco-1088-04 was sampled in 2004. Where maximum-likelihood tree was congruent with maximum parsimony tree, branch lengths are indicated below branches. Number of homoplasies is indicated after the branch length. Bootstrap values are shown (maximum parsimony/minimum evolution/maximum likelihood). The score (–ln likelihood) of the best maximum-likelihood tree was env 3615.01706, LTRs 1836.05922 (best tree found by maximum parsimony: env length = 221, consistency index [CI] = 0.941, retention index [RI] = 0.963; LTR length = 132, CI = 0.871, RI = 0.787).

Figure 4. Phylogenetic trees of panther feline leukemia virus (FeLV-Pco) and domestic cat FeLV nucleotide sequences. A) Midpoint rooted maximum-likelihood phylogram based on 1,698 bp of env sequences. B) Midpoint rooted maximum-likelihood phylogram based on 463 bp of 3’ long terminal repeat (LTR) sequences. Consensus FeLV-Pco sequences of clones generated from 5 env and 4 LTR panthers and reference domestic cat sequences are shown. The number of FeLV-Pco–cloned PCR products used in each consensus sequence is indicated in parentheses. The arrow indicates the monophyletic clade of all FeLV-Pco sequences. A similar topology, including the monophyletic clade, was obtained by using the different FeLV-Pco clone sequences rather than a consensus. The year of panther sampling is indicated as a suffix, e.g., Pco-1088-04 was sampled in 2004. Where maximum-likelihood tree was congruent with maximum parsimony tree, branch lengths are indicated below branches. Number of homoplasies is indicated after the branch length. Bootstrap values are shown (maximum parsimony/minimum evolution/maximum likelihood). The score (–ln likelihood) of the best maximum-likelihood tree was env 3615.01706, LTRs 1836.05922 (best tree found by maximum parsimony: env length = 221, consistency index [CI] = 0.941, retention index [RI] = 0.963; LTR length = 132, CI = 0.871, RI = 0.787).

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