Volume 14, Number 3—March 2008
Research
Molecular Epidemiology of Eastern Equine Encephalitis Virus, New York
David S. Young*, Laura D. Kramer*†, Joseph G. Maffei*, Robert J. Dusek‡, P. Bryon Backenson*†, Christopher N. Mores§, Kristen A. Bernard*†, and Gregory D. Ebel¶
Table A1
Eastern equine encephalitis virus strains collected during the current and past epizootics, USA
| Strain Designation* | Strain | Location | Date Collected | Source | Passage History† | Accession no. |
|
|---|---|---|---|---|---|---|---|
| E2 | NSP3 | ||||||
| VA33 | TenBroeck | Virginia | 1933 | Horse | sm12, v1 | U01558 | |
| MA38 | Massachusetts | Massachusetts | 1938 | Human | unknown | AF159550 | |
| LA47 | Decuir | Louisiana | 1947 | Human | P1 | U01552 | |
| LA50 | Arth-167 | Louisiana | 1950 | Mosquito | gp2, ch2 | AF159551 | |
| Brazil56 | BeAn-5122 | Brazil | 1956 | Sentinel monkey | Sm2 | AF159559 | |
| NJ60 | New Jersey 60 | New Jersey | 1960 | Mosquito | p6, sm1 | U01554 | |
| NY69 | NY69-7836 | Suffolk, NY | 1969 | Pheasant | Unknown | EF594003 | EF593961 |
| NY71a | NY71-3465 | Oswego, NY | 8/23/1971 | Horse | Unknown | EF594004 | EF593962 |
| NY71b | NY71-20686 | Oswego, NY | 8/24/1971 | Zonotrichia albicollis | Unknown | EF594005 | EF593963 |
| NY71c | NY71-20925 | Oswego, NY | 8/28/1971 | Culex restuans | Unknown | EF594006 | EF593964 |
| NY73 | NY73-60104 | Onondaga, NY | 10/1/1973 | Horse | Unknown | EF594007 | EF593965 |
| NY74a | NY74-39071 | Oswego, NY | 8/26/1974 | Horse | Unknown | EF594008 | EF593966 |
| NY74b | NY74-39073 | Oswego, NY | 9/2/1974 | Horse | Unknown | EF594009 | EF593967 |
| NY74c | NY74-23179 | Oswego, NY | 9/4/1974 | Melospiza melodia | Unknown | EF594010 | EF593968 |
| NY74d | NY74-33620 | Oswego, NY | 9/5/1974 | Culiseta melanura | Unknown | EF594011 | EF593969 |
| NY74e | NY74-33621 | Oswego, NY | 9/5/1974 | Aedes canadensis | Unknown | EF594012 | EF593970 |
| NY74f | NY74-33630 | Oswego, NY | 9/12/1974 | Ae. canadensis | Unknown | EF594013 | EF593971 |
| NY74g | NY74-33927 | Oswego, NY | 9/14/1974 | Cs. melanura | Unknown | EF594014 | EF593972 |
| NY74h | NY74-39100 | Oswego, NY | 9/20/1974 | Horse | Unknown | EF594015 | EF593973 |
| NY75 | NY75-39011 | Oswego, NY | 7/16/1975 | Horse | unknown | EF594016 | EF593974 |
| MA77 | ME77132 | Massachusetts | 1977 | Mosquito | m1, C6/36-1 | U01555 | |
| WI80 | WiAn-5000 | Wisconsin | 1980 | Horse | de2, sm1, v1 | U01559 | |
| FL82 | 82V-2137 | Florida | 1982 | Mosquito | Unknown | U01034 | U01034 |
| MS83 | MS-4789 | Mississippi | 1983 | Human | rd2, sm3 | AF159552 | |
| MD85 | 215-85 | Maryland | 1985 | Mosquito | bhk1 | U01556 | |
| Brazil85 | 436087 | Brazil | 1985 | Mosquito | sm1,v1 | AF159561 | |
| Panama86 | 435731 | Panama | 1986 | Horse | v2 | AF159560 | |
| CT90 | Williams | Connecticut | 1990 | Horse | v1 | U01557 | |
| MD90 | 3067-90 | Maryland | 1990 | Mosquito | Unpassed | U01553 | |
| FL91 | Florida91-4697 | Florida | 1991 | Mosquito | sm1, v3, bhk2 | AY705241 | AY705241 |
| GA91 | PorEEE | Georgia | 1991 | Pig | Unknown | AF159557 | |
| TX91 | VRI-7164 | Texas | 1991 | Horse | sm1 | AF159553 | |
| FL93 | FL93-939 | Florida | 1993 | Mosquito | v1 | AF159554 | |
| TX95 | PV5-2547C | Texas | 1995 | Mosquito | sm1 | AF159555 | |
| FL96 | FL96-14834 | Florida | 1996 | Bird | v1 | AF159556 | |
| GA97 | Georgia 97 | Georgia | 1997 | Human | v2 | AY705240 | AY705240 |
| Mexico97 | 97-1076 | Mexico | 1997 | Horse | v1 | AF159558 | |
| LA02 | LA02-5534 | Louisiana | 9/28/2002 | Thryothorus ludovicianus | v1 | EF593999 | EF593957 |
| FL02a | FL02-4341 | Florida | 10/7/2002 | Vireo griseus | v1 | EF593997 | EF593955 |
| FL02b | FL02-4809 | Florida | 10/16/2002 | Seiurus aurocapillus | v1 | EF593998 | EF593956 |
| NJ03a | NJ03-1974 | New Jersey | 9/24/2003 | Empidonax traillii/alnorum | v1 | EF594000 | EF593958 |
| NJ03b | NJ03-1977 | New Jersey | 9/24/2003 | Passerina cyanea | v1 | EF594001 | EF593959 |
| VA03 | VA03-2789 | Virginia | 9/30/2003 | Vireo olivaceus | v1 | EF594002 | EF593960 |
| NY03a | NY03-0127 | Suffolk, NY | 8/26/2003 | Cs. melanura | Unpassed | EF594017 | EF593975 |
| NY03b | NY03-2657 | Orange, NY | 9/2/2003 | Corvus brachyrhynchos | Unpassed | EF594018 | EF593976 |
| NY03c | NY03-0248 | Onondaga, NY | 9/11/2003 | Cs. melanura | Unpassed | EF594019 | EF593977 |
| NY03d | NY03-0199 | Onondaga, NY | 9/25/2003 | Cs. melanura | Unpassed | EF594020 | EF593978 |
| NY04a | NY04-0347 | Onondaga, NY | 7/20/2004 | Cs. melanura | Unpassed | EF594021 | EF593979 |
| NY04b | NY04-0390 | Onondaga, NY | 7/29/2004 | Cs. melanura | Unpassed | EF594022 | EF593980 |
| NY04c | NY04-0391 | Onondaga, NY | 8/3/2004 | Cs. melanura | Unpassed | EF594023 | EF593981 |
| NY04d | NY04-0384 | Onondaga, NY | 8/5/2004 | Cs. melanura | Unpassed | EF594024 | EF593982 |
| NY04e | NY04-0340 | Onondaga, NY | 8/26/2004 | Cs. melanura | Unpassed | EF594025 | EF593983 |
| NY04f | NY04-0401 | Onondaga, NY | 9/1/2004 | Cs. melanura | Unpassed | EF594026 | EF593984 |
| NY04g | NY04-2073 | Sullivan, NY | 9/7/2004 | Haliaeetus leucocephalus | Unpassed | EF594027 | EF593985 |
| NY04h | NY04-0344 | Onondaga, NY | 9/9/2004 | Cs. melanura | Unpassed | EF594028 | EF593986 |
| NY04i | NY04-2727 | Onondaga, NY | 9/14/2004 | Corvus brachyrhynchos | Unpassed | EF594029 | EF593987 |
| NY04j | NY04-0029 | Ulster, NY | 9/16/2004 | Horse | Unpassed | EF594030 | EF593988 |
| NY04k | NY04-0037 | Chemung, NY | 10/10/2004 | Horse | Unpassed | EF594031 | EF593989 |
| NY05a | NY05-0404 | Onondaga, NY | 7/14/2005 | Cs. melanura | v1 | EF594032 | EF593990 |
| NY05b | NY05-0411 | Onondaga, NY | 7/26/2005 | Coquillettidia perturbans | Unpassed | EF594033 | EF593991 |
| NY05c | NY05-0443 | Onondaga, NY | 7/26/2005 | Cs. melanura | Unpassed | EF594034 | EF593992 |
| NY05d | NY05-0410 | Madison, NY | 8/24/2005 | Cs. melanura | v1 | EF594035 | EF593993 |
| NY05e | NY05-2433 | Onondaga, NY | 9/28/2005 | Cyanocitta cristata | Unpassed | EF594036 | EF593994 |
| NY05f | NY05-0050 | Oswego, NY | 10/5/2005 | Horse | Unpassed | EF594037 | EF593995 |
| Ten Broeck‡ | EEE-Ten Broeck | Virginia | 1933 | Horse | Unknown | EF594038 | EF593996 |
*Strain designations in bold were sequenced in this study, GenBank sequences from Brault et al. (1999) and Platteborze et al. (2005) (34,35).
†Passage history obtained from Brault et al. (1999) and Platteborze et al. (2005) (34,35); v, Vero cell culture; sm, suckling mouse; gp, guinea pig; ch, chicken embryo; p, unknown passages; C6/36, C636 clone of Aedes albopictus cell culture; bhk, baby hamster kidney cell culture; de, duck embryo cell culture; rd, human embryonal rhabdomyosarcoma cell culture; m, mosquito.
‡Information uncertain for Ten Broeck strain sequenced in this study, thought to be derived from passaged VA33 strain.
