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Volume 14, Number 4—April 2008

Research

Rapid Typing of Transmissible Spongiform Encephalopathy Strains with Differential ELISA

Stéphanie Simon*, Jérôme Nugier*, Nathalie Morel*, Hervé Boutal*, Christophe Créminon*, Sylvie L. Benestad†, Olivier Andréoletti‡, Frédéric Lantier§, Jean-Marc Bilheude¶, Muriel Feyssaguet¶, Anne-Gaëlle Biacabe#, Thierry Baron#, and Jacques Grassi*Comments to Author 
Author affiliations: *Commissariat à l’Energie Atomique, Gif-sur-Yvette, France; †National Veterinary Institute, Oslo, Norway; ‡Institut National de la Recherche Agronomique-Ecole Nationale Vétérinaire de Toulouse, Toulouse, France; §Infectiologie Animale et Santé Publique, Tours, France; ¶Bio-Rad, Marnes-la-Coquette, France; #Agence Française de Sécurité Sanitaire des Aliments, Lyon, France;

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Table 1

Analysis of the reproducibility of the ELISA typing test*

Sample (N = 20) Mean ratio A/A′(normalized) SD (normalized) CV, % 95% CI (mean ± 2× SD)
Manual protocol        
Classic scrapie 99-1316 1.3 (0.13) 0.2 (0.02) 15.4 0.9–1.7 (0.09–0.18)
Intermediate scrapie PG1259 4.5 (0.46) 0.8 (0.08) 17.8 2.9–6.1 (0.30–0.63)
Experimental ovine BSE 397BS 9.7 (1.00) 2.0 (0.21) 20.6 5.7–13.7 (0.59–1.41)
Automated protocol        
Classic scrapie 99-1316 1.6 (0.25) 0.2 (0.03) 13.7 1.2–1.9 (0.19–0.30)
Intermediate scrapie 99-1487 3.8 (0.59) 0.5 (0.08) 13.4 2.8–4.8 (0.44–0.75)
Experimental ovine BSE 397BS 6.4 (1.00) 0.9 (0.14) 14.8 4.5–8.3 (0.70–1.30)

*Three samples (classic scrapie 99-1316, intermediate scrapie PG1259 or 99-1487, and experimental ovine BSE 397BS) were analyzed in 20 different experiments using the manual or automated protocol (see Experimental Procedures). Normalized ratios are indicated in parentheses. SD, standard deviation; CV, coefficient of variation; CI, confidence interval; BSE, bovine spongiform encephalopathy.

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