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Volume 14, Number 7—July 2008

Dispatch

Molecular Typing of Trypanosoma cruzi Isolates, United States

Dawn M. Roellig*Comments to Author , Emily L. Brown*, Christian Barnabé†, Michel Tibayrenc†, Frank J. Steurer‡, and Michael J. Yabsley*
Author affiliations: *University of Georgia, Athens, Georgia, USA; †Institut de Recherche pour le Developpement, Montpellier, France; ‡Centers for Disease Control and Prevention, Atlanta, Georgia, USA;

Main Article

Table

Approximate amplicon sizes of gene targets and lineage determination in Trypanosoma cruzi

Strain Mini-exon, bp 24S α rRNA, bp 18S rRNA, bp Lineage
FL Opo 15* 350 110 175 I
GA Rac 103* None 120 155 IIa
FL Rac 5* 400 120 155 IIa
93053103R cl3 350 110 175 I
FL Rac 13 350 110, 120 155, 175 I/IIa†
FL Rac 46 400 110, 120 155 I/IIa†
Griffin Dog 350 110, 120 155 I/IIa†
Monk RH89–40 None 110 155 I/IIa†

*Denotes isolates used as representative banding patterns seen for classic lineage typing.
†Because of atypical banding patterns, a clear definition of an isolate as type I vs. type IIa could not be obtained.

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