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Volume 14, Number 8—August 2008

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Analysis of Collection of Hemolytic Uremic Syndrome–associated Enterohemorrhagic Escherichia coli

Alexander Mellmann*Comments to Author , Martina Bielaszewska*, Robin Köck*, Alexander W. Friedrich*, Angelika Fruth†, Barbara Middendorf*, Dag Harmsen‡, M. Alexander Schmidt§, and Helge Karch*
Author affiliations: *Institute for Hygiene and the National Consulting Laboratory on Hemolytic Uremic Syndrome, Münster, Germany; †Robert Koch Institute, Wernigerode, Germany; ‡Periodontology, Münster; §Center for Molecular Biology of Inflammation, Münster;

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Appendix Figure


Minimum spanning tree of hemolytic uremic syndrome–associated enterohemorrhagic Escherichia coli strains and Shigella dysenteriae M1354 (ST243, data from the E. coli multilocus sequence type [ST] website [http://web.mpiib-berlin.mpg.de/mlst/dbs/Ecoli]) as an outgroup generated from allelic profiles based on the eBURST algorithm (12). Each ST is represented by a circle named with its ST, and the corresponding serotypes are given (OR, O rough; H–, nonmotile; nt, not typeable with the E. coli O and H antisera used). Black lines connecting pairs of STs indicate that they share 6 (thick lines) or 5 (thin) alleles. Gray lines connecting pairs of STs of increasing line length indicate that the STs share <4 alleles. In addition, the STs and, if applicable, the connecting lines of a clonal complex are shaded in gray.

Appendix Figure. 
Minimum spanning tree of hemolytic uremic syndrome–associated enterohemorrhagic Escherichia coli strains and Shigella dysenteriae M1354 (ST243, data from the E. coli multilocus sequence type [ST] website [http://web.mpiib-berlin.mpg.de/mlst/dbs/Ecoli]) as an outgroup generated from allelic profiles based on the eBURST algorithm (12). Each ST is represented by a circle named with its ST, and the corresponding serotypes are given (OR, O rough; H, nonmotile; nt, not typeable with the E. coli O and H antisera used). Black lines connecting pairs of STs indicate that they share 6 (thick lines) or 5 (thin) alleles. Gray lines connecting pairs of STs of increasing line length indicate that the STs share <4 alleles. In addition, the STs and, if applicable, the connecting lines of a clonal complex are shaded in gray.

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