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Volume 14, Number 8—August 2008

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Endemic Circulation of European Bat Lyssavirus Type 1 in Serotine Bats, Spain

Sonia Vázquez-Morón*†Comments to Author , Javier Juste‡, Carlos Ibáñez‡, Eduardo Ruiz-Villamor§, Ana Avellón*, Manuel Vera*, and Juan E. Echevarría*†
Author affiliations: *Instituto de Salud Carlos III, Majadahonda, Madrid, Spain; †Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública (CIBERESP), Spain; ‡Estación Biológica de Doñana CSIC, Sevilla. Spain; §Laboratorio Central de Veterinaria de Santa Fe, Granada, Spain;

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Figure 2

Figure 2 - Pathologic images obtained from a carcass of Eptesicus isabellinus. The bat was captured while flying but died during handling. Brain specimen was positive for lyssavirus antigens by immunofluorescence and for European bat lyssavirus 1 RNA by reverse transcription–PCR. A) Neural degeneration in brain by hematoxylin and eosin stain (H&E); magnification ×400. B) Negative Seller stain in spinal cord indicating the absence of Negri bodies; magnification ×400. C) Positive Feu

Figure 2. Pathologic images obtained from a carcass of Eptesicus isabellinus. The bat was captured while flying but died during handling. Brain specimen was positive for lyssavirus antigens by immunofluorescence and for European bat lyssavirus 1 RNA by reverse transcription–PCR. A) Neural degeneration in brain by hematoxylin and eosin stain (H&E); magnification ×400. B) Negative Seller stain in spinal cord indicating the absence of Negri bodies; magnification ×400. C) Positive Feulgen reaction in brain, glial cell neurophagia; magnification ×200. D) Focal proliferation of glial cells by H&E; magnification ×100.

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