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Volume 14, Number 8—August 2008

Research

Genetic and Serologic Properties of Zika Virus Associated with an Epidemic, Yap State, Micronesia, 2007

Robert S. Lanciotti*Comments to Author , Olga L. Kosoy*, Janeen J. Laven*, Jason O. Velez*, Amy J. Lambert*, Alison J. Johnson*, Stephanie M. Stanfield*, and Mark R. Duffy*
Author affiliations: *Centers for Disease Control and Prevention, Fort Collins, Colorado, USA;

Main Article

Table 4

Results of quantitative real-time RT-PCR of samples from ZIKV-positive patients, Yap State, Micronesia, 2007*

PatientDays after onsetZIKV real-time RT-PCR
Ct-860†Ct-1107†ResultEstimated copies/mL‡
824134.334.7+11,647
939232.032.4+67,817
947234.333.9+21,495
949235.135.1+8,573
969129.429.3+728,800
037132.132.5+62,816
830a230.730.0+426,325
847a534.834.7+11,647
950a032.232.7+53,894
943337.635.6+5,845
952129.329.5+625,280
9581129.930.3+338,797
970135.534.8+10,788
42032.933.6+27,048
941331.138.0+930
964038.337.6+1,263
063a237.538.0+930

*RT-PCR, reverse transcription­–PCR; ZIKV, Zika virus; Ct, crossing threshold; +, positive.
†Ct values with primer set 835/911c/860-FAM or 1086/1162c/1107-FAM. Values <38.5 are positive.
‡Estimated by testing quantitated dilutions of ZIKV RNA transcripts and standard curve calculation generated by the iCycler instrument (Bio-Rad, Hercules, CA, USA; see Methods).

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