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Volume 15, Number 1—January 2009

Letter

SCCmec Typing in Methicillin-Resistant Staphylococcus aureus Strains of Animal Origin

Marc D. Jansen, Adrienne T.A. BoxComments to Author , and Ad C. FluitComments to Author 
Author affiliations: University Medical Center Utrecht, Utrecht, the Netherlands

Main Article

Table

Primers used to type SCCmec of MRSA ST398 isolates*

Genes Primer name Primer sequence (5′ → 3′)
ccrA/B1 ccr1B-for CTT TCA CGA TAG ACA CAG

ccr1B-rev
TAA AAG AAG TTC ATA GCC GTT AAA TTG G
ccrA/B2 ccr2B-for GCA TTC ATC ATC AAT CAA AAT G

ccr2B-rev
CTA TAA CCT TCT GTG CTT TGC A
ccrA/B3 ccr3B-for TCC GTA ATA AGA AGC AAC TTC AC

ccr3B-rev
ACT ATA GCC TTC AGT ACT TTG GA
ccrA/B4 ccr4B-for TGA AGA AGC ACA AGA GCG GC
ccr4B-rev CTG CAC CAC ATT TTG GGC AC

*SCCmec, staphylococcal cassette chromosome mec; MRSA, methicillin-resistant Staphylococcus aureus; ST, sequence type.

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