Volume 15, Number 1—January 2009
Rickettsia helvetica in Dermacentor reticulatus Ticks
|Species||Primers and probe||Sequence (5′ → 3′)|
*Amplified products: 162 bp (R. helvetica); 228 bp (R. slovaca); 646 bp (D. reticulatus); standard curve: slope; Y-intercept and correlation coefficient:
–3.634, 40.129, 0.9937 (R. helvetica); –0.23, 42.82, 0.9942 (R. slovaca); ITS, internal transcribed spacer.
†The TaqMan probes were labeled with the fluorescent dyes FAM at the 5' end and TAMRA as quencher at the 3' end.
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