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Volume 15, Number 11—November 2009

Research

Epidemiology of Hepatitis A Virus Infections, Germany, 2007–2008

Mirko S. Faber, Klaus Stark, Susanne C. Behnke, Eckart Schreier, and Christina FrankComments to Author 
Author affiliations: Robert Koch Institute, Berlin, Germany

Main Article

Table 1

Primers used for detection of HAV RNA by nested RT-PCR in clinical specimens from patients in Germany, 2007–2008*

Primer Sequence (5′ → 3′) Orientation, position, and use
HAV6a ggA AAT ATT CAg ATT Agg YTg CCT Tgg T Sense, 2793–2820, reverse transcription and first-round PCR
HAV6b ggg AAC ATT CAg ATY AgA TTg CCW Tgg T
HAV17a CAA AgC TCT AgT RTC AgC AgT AAT TCC Antisense, 3300–3326, reverse transcription and first-round PCR
HAV17b CAA AgC CCT AgT RTC AgC AgT CAC TCC
HAV8a CTT TTg gAT TKg TTT CYA TTC AgA TTg C Sense, 2882–2908, nested PCR and sequencing
HAV7a gAA AAC TTC ATT ATT TCA TgM TCY TCW gT Antisense, 3264–3292, nested PCR and sequencing

*HAV, hepatitis A virus; RT-PCR, reverse transcription–PCR. Positions in the HAV genome are given according to strain HM-175 (GenBank accession no. M14707).

Main Article

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