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Volume 15, Number 11—November 2009

Letter

Rickettsia massiliae in the Canary Islands

Isabel G. Fernández de Mera, Zorica Zivkovic, Margarita Bolaños, Cristina Carranza, José Luis Pérez-Arellano, Carlos Gutiérrez, and José de la FuenteComments to Author 
Author affiliations: Instituto de Investigación en Recursos Cinegéticos IREC (Consejo Superior de Investigaciones Cientificas–Universidad de Castilla-La Mancha–Junta de Comunidades de Castilla-La Mancha), Ciudad Real, Spain (I.G. Fernández de Mera, J. de la Fuente); Utrecht University, the Netherlands (Z. Zivkovic); Hospital Universitario Insular de Gran Canaria, Canary Islands, Spain (M. Bolaños, C. Carranza, J.L. Pérez-Arellano); Universidad de Las Palmas de Gran Canaria, Canary Islands (J.L. Pérez-Arellano, Carlos Gutiérez); Oklahoma State University, Stillwater, Oklahoma, USA (J. de la Fuente).

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Table

Rickettsia massiliae PCR conditions and amplicon size, Canary Islands, 2008*

Gene Description
(GenBank accession no.) Primer sequence (5′ → 3′) Amplicon size, bp PCR annealing conditions
16S rRNA 16S ribosomal RNA
(GQ144453) F: AGAGTTTGATCCTGGCTCAG
R: AACGTCATTATCTTCCTTGC 416 50°C/30 s
ompB Outer membrane protein (GQ144450) F: GGGTGCTGCTACACAGCAGAA
R: CCGTCACCGATATTAATTGCC 618 53°C/30 s
dnaK Heat-shock protein 70
(GQ144451) F: AGCGTCAAGCAACGAAAGAT
R: CAAACGTTGAAGTGCTAAAGG 323 50°C/30 s
dnaA Chromosomal replication initiation protein (GQ144449) F: CCTACTAACTTTGTTAGAGATT
R: TGATGATTCTGCAACCGCTC 241 56°C/30 s
recA RecA recombination protein (GQ144452) F: TGCTTTTATTGATGCCGAGC
R: CTTTAATGGAGCCGATTCTTC 428 52°C/30 s
atpA ATP synthase F1 alpha subunit (GQ144448) F: ACATATCGAGATGAAGGCTCC
R: CCGAAATACCGACATTAACG 731 48°C/30 s

*GenBank accession numbers correspond to R. massiliae sequences identified in this study. PCRs were completed by employing the Access RT-PCR system (Promega, Madison, WI, USA) with 1 ng DNA, the oligonucleotide primers, and annealing conditions and with extension for 1 min at 68ºC. F, forward; R, reverse.

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