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Volume 15, Number 12—December 2009

Dispatch

Diagnostic Assay for Rickettsia japonica

Nozomu Hanaoka, Minenosuke Matsutani, Hiroki Kawabata, Seigo Yamamoto, Hiromi Fujita, Akiko Sakata, Yoshinao Azuma, Motohiko Ogawa, Ai Takano, Haruo Watanabe, Toshio Kishimoto, Mutsunori Shirai, Ichiro Kurane, and Shuji AndoComments to Author 
Author affiliations: National Institute of Infectious Diseases, Tokyo, Japan (N. Hanaoka, H. Kawabata, A. Sakata, M. Ogawa, A. Takano, H. Watanabe, T. Kishimoto, I. Kurane, S. Ando); Yamaguchi University School of Medicine, Yamaguchi, Japan (M. Matsutani, Y. Azuma, M. Shirai); Miyazaki Prefectural Institute for Public Health and Environment, Miyazaki, Japan (S. Yamamoto); Ohara General Hospital, Fukushima, Japan (H. Fujita); Gifu University, Gifu, Japan (H. Kawabata, A. Takano, H. Watanabe)

Main Article

Table 1

Reactivity of the real-time PCR for Rickettsia strains used in this study*

Species Strain Isolation source Reference Real-time PCR
Rickettsia asiatica IO-1 Ixodes ovatus ATCC VR-1593 (8)
IO-2 I. ovatus (8)
IO-3 I. ovatus (8)
IO-25 I. ovatus (8)

IO-38
I. ovatus
(8)

R. conorii
Malish 7
Human
ATCC VR 613T

R. heilongjiangensis
CH8-1
Haemaphysalis concinna
(6)
+
R. helvetica IM-1 Ixodes monospinosus (9)
IP-1 I. persulcatus (9)
IP-2 I. persulcatus (10)

IP-6
I. persulcatus
This study

R. honei
TT-118
Ixodes sp.
(11)

R. japonica DT-1 Dermacentor taiwanensis (12) +
YH Human ATCC VR-1363 +
FLA-1 Haemaphysalis flava (9) +
HH-8 H. hystricis This study +

HH-9
H. hystricis
This study
+
R. prowazekii
breinl
Human
(13)

R. rickettsii
Sheila Smith
Human
(14)

R. sibirica
246
Human
ATCC VR-151

R. tamurae AT-1 Amblyomma testudinarium ATCC VR-1594 (12)
AT-4 A. testudinarium (6)

AT-13
A. testudinarium
(6)

R. typhi
Wilmington
Human
ATCC VR-144

Rickettsia sp. LON LON-2 Haemaphysalis longicornis (6)
LON-9 H. longicornis (6)
LON-13 H. longicornis (6)

*+, positive; –, not detected.

Main Article

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