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Volume 15, Number 12—December 2009

Dispatch

Diagnostic Assay for Rickettsia japonica

Nozomu Hanaoka, Minenosuke Matsutani, Hiroki Kawabata, Seigo Yamamoto, Hiromi Fujita, Akiko Sakata, Yoshinao Azuma, Motohiko Ogawa, Ai Takano, Haruo Watanabe, Toshio Kishimoto, Mutsunori Shirai, Ichiro Kurane, and Shuji AndoComments to Author 
Author affiliations: National Institute of Infectious Diseases, Tokyo, Japan (N. Hanaoka, H. Kawabata, A. Sakata, M. Ogawa, A. Takano, H. Watanabe, T. Kishimoto, I. Kurane, S. Ando); Yamaguchi University School of Medicine, Yamaguchi, Japan (M. Matsutani, Y. Azuma, M. Shirai); Miyazaki Prefectural Institute for Public Health and Environment, Miyazaki, Japan (S. Yamamoto); Ohara General Hospital, Fukushima, Japan (H. Fujita); Gifu University, Gifu, Japan (H. Kawabata, A. Takano, H. Watanabe)

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Table 2

Application of PCRs for blood clot specimens derived from acute-stage Japanese spotted fever patients*

Patient no. Age, y/sex Days after onset of fever† Laboratory examinations
Real-time PCR¶
Rickettsia isolation‡ Serodiagnosis§ Conventional PCR
Rj5–Rj10 assay R1–R2 assay
C1 83/F 5 + + 38.2 ± 0.6
C2 35/F 6 + +
C3 70/M 6 + + 37.6 ± 1.3
C4 71/M 3 + +
C5 66/F 6 + + 38.4 ± 1.0
C6 49/M 3 NT +
C7 88/F 5 NT + 40.7 ± 0.4
C8 49/M 7 NT + 31.4 ± 1.1
C9 65/F 3 NT + 36.0 ± 0.5
C10 78/M 4 NT + 39.0 ± 0.8
C11 72/F 4 NT +
C12 68/F 5 NT +
C13 27/M 5 NT +
C14 45/M 7 NT +
C15 76/F 5 NT + 39.0 ± 0.5
C16 79/M 3 NT + 39.9 ± 1.0
C17 69/M 2 NT +
C18 64/F 3 NT +

*+, positive; NT, not tested; –, not detected.
†Blood clot was prepared from whole blood at indicated days after onset of fever.
‡Isolation from whole blood specimens.
§Seroconversion was identified by the indirect immunofluorescent test with paired serum specimens.
¶Cycle threshold values are given as means ± standard errors of the means for 3 independent assays.

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