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Volume 15, Number 12—December 2009

Dispatch

Transplacental Transmission of Bluetongue Virus 8 in Cattle, UK

Karin E. DarpelComments to Author , Carrie A. Batten, Eva Veronesi, Susanna Williamson, Peter Anderson, Mike Dennison, Stuart Clifford, Ciaran Smith, Lucy Philips, Cornelia Bidewell, Katarzyna Bachanek-Bankowska, Anna Sanders, Abid Bin-Tarif, Anthony J. Wilson, Simon Gubbins, Peter P.C. Mertens, Chris A. Oura, and Philip S. Mellor
Author affiliations: Institute for Animal Health, Pirbright, UK (K.E. Darpel, C.A. Batten, E. Veronesi, K. Bachanek-Bankowska, A. Sanders, A. Bin-Tarif, A.J. Wilson, S. Gubbins, P.P.C. Mertens, C.A. Oura, P.S. Mellor); Veterinary Laboratories Agency, Bury St. Edmunds, UK (S. Williamson, C. Bidewell); Animal Health Divisional Office, Bury St. Edmunds (P. Anderson, S. Clifford, C. Smith, L. Philips); Animal Health Divisional Office, Chelmsford, UK (M. Dennison)

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Table

Bluetongue virus real-time reverse transcription–PCR results from follow-up sampling of calves with initial positive results, United Kingdom, December 20, 2007, to March 15, 2008*

Calf no. First BTV result, age, d (Ct) Retest results, age, d (Ct)
Age, d, when PCR negative Estimated gestation, d†
Retest 1 Retest 2 Retest 3 Retest 4 Retest 5
1 15 (25) 28 (26) 44 (26) 58 (28.5) 72 (32.5) 91 (neg) 91 82–219
3 38 (31) 47 (32) 61 (35.5) 81 (neg) NT NT 81 106–243
10 79 (32) 106 (33.5) 120 (34) 137 (neg) 158 (neg) NT 137 140–197
12 81 (28) 108 (30) 122 (31) 139 (34) 160 (neg) NT 160 142–199
13 4 (33) 31 (36.5) 45 (neg) 62 (neg) 83 (neg) NT 45 65–122
14 28 (26) 48 (29) 55 (32) 69 (neg) 86 (neg) 107 (neg) 69 154–209
15 70 (32) 97 (neg) 111 (neg) 128 neg) 149 (neg) NT 97 196–251
20 17 (31) 44 (32.5) 58 (33.5) 75 (neg) 96 (neg NT 75 78–128
25 27 (29.5) 41 (29) 55 (30.5) 69 (36) NT NT >69‡ 145–202
28 1 (23) 26 (25) 35 (26) NT NT >35‡ 101–181
29 1 (27) 12 (27.5) Calf died 45–182
41 47 (28) 61 (29.5) NT NT NT NT >61‡ 79–126
45 22 (27) 40 (30.5) 61 (34) NT NT NT >61‡ 52–130
47 25 (26.5) 39 (29) 66 (38) NT NT NT >66‡ 52–189
49 (twin with 50) 46 (29) 60 (36) 87 (neg) NT NT NT 87 73–136
50 (twin with 49) 46 (29) 60 (36.5) 87 (neg) NT NT NT 87 73–136
55 21 (25.5) 48 (31.5) NT NT NT >48‡ 34–172

*BTV, bluetongue virus; Ct, cycle threshold; neg, negative; NT, not tested.
†Estimated stage of gestation at which transplacental infection may have occurred
‡These calves could not be followed up for farm management reasons or because the project had ended.

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