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Volume 15, Number 2—February 2009

Research

Bacterial Phenotype Variants in Group B Streptococcal Toxic Shock Syndrome1

Parham Sendi2Comments to Author , Linda Johansson, Samira Dahesh, Nina M. Van Sorge, Jessica Darenberg, Mari Norgren, Jan Sjölin, Victor Nizet, and Anna Norrby-Teglund
Author affiliations: Karolinska Institutet, Stockholm, Sweden (P. Sendi, L. Johansson, A. Norrby-Teglund); Basel University Medical Clinic, Liestal, Switzerland (P. Sendi); University of California San Diego, La Jolla, California, USA (S. Dahesh, N.M. Van Sorge, V. Nizet); Swedish Institute for Infectious Disease Control, Stockholm (J. Darenberg); Umeå University, Umeå, Sweden (M. Norgren); Uppsala University, Uppsala, Sweden (J. Sjölin)

Main Article

Figure 1

A) Hemolytic zone on blood agar plate after 48 h: Low hemolytic (LH) colony and high hemolytic (HH) colony. B) Hemolytic activity of the pigment extract presented as hemolytic capacity (left graph) relative to that of sodium dodecyl sulfate (100%) and as hemolytic titer (right graph) evaluated with a microdilution assay. Error bars indicate SEM. C) Phenotypic appearance of group B streptococci after overnight culture in Todd-Hewitt broth plus 1.5% yeast extract, displaying a white pellet (LH) an

Figure 1. A) Hemolytic zone on blood agar plate after 48 h: Low hemolytic (LH) colony and high hemolytic (HH) colony. B) Hemolytic activity of the pigment extract presented as hemolytic capacity (left graph) relative to that of sodium dodecyl sulfate (100%) and as hemolytic titer (right graph) evaluated with a microdilution assay. Error bars indicate SEM. C) Phenotypic appearance of group B streptococci after overnight culture in Todd-Hewitt broth plus 1.5% yeast extract, displaying a white pellet (LH) and an orange pellet (HH). D) Absorbance profile of the pigment extract. E) Results of CAMP testing, which display a stronger reaction with the LH than with the HH phenotype.

Main Article

1Part of this work was presented at Lancefield 2008 International Symposium on Streptococci and Streptococcal Diseases, Porto Heli, Greece, June 22–26, 2008.

2Current affiliation: Clinic for Infectious Diseases, University Hospital Bern, Bern, Switzerland.

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