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Volume 15, Number 2—February 2009

Research

Bacterial Phenotype Variants in Group B Streptococcal Toxic Shock Syndrome1

Parham Sendi2Comments to Author , Linda Johansson, Samira Dahesh, Nina M. Van Sorge, Jessica Darenberg, Mari Norgren, Jan Sjölin, Victor Nizet, and Anna Norrby-Teglund
Author affiliations: Karolinska Institutet, Stockholm, Sweden (P. Sendi, L. Johansson, A. Norrby-Teglund); Basel University Medical Clinic, Liestal, Switzerland (P. Sendi); University of California San Diego, La Jolla, California, USA (S. Dahesh, N.M. Van Sorge, V. Nizet); Swedish Institute for Infectious Disease Control, Stockholm (J. Darenberg); Umeå University, Umeå, Sweden (M. Norgren); Uppsala University, Uppsala, Sweden (J. Sjölin)

Main Article

Figure 3

A) Difference in pigmentation of the low hemolytic (LH)/high encapsulation (HC) and NEM316 wild-type (WT) strains and their corresponding transformants expressing the covR/S locus of the high hemolytic (HH)/low encapsulation (LC) variant after overnight culture. B) CAMP testing with strains displayed according to panel A. The LH/HC variant and the NEM316 strain display a stronger reaction (arrows) than their corresponding transformants. C) Semiquantitative analysis of mRNA expression of CAMP fac

Figure 3. A) Difference in pigmentation of the low hemolytic (LH)/high encapsulation (HC) and NEM316 wild-type (WT) strains and their corresponding transformants expressing the covR/S locus of the high hemolytic (HH)/low encapsulation (LC) variant after overnight culture. B) CAMP testing with strains displayed according to panel A. The LH/HC variant and the NEM316 strain display a stronger reaction (arrows) than their corresponding transformants. C) Semiquantitative analysis of mRNA expression of CAMP factor (cftb), β-h/c (cylK and cylE) capsule (cpsG), and gyrA (housekeeping gene) using reverse transcription–PCR. Lane 1, LH/HC; lane 2, HH/LC; lane 3, LH/HC + pcovR HH; lane 4, NEM316 WT; lane 5, NEM316 + pcovR HH.

Main Article

1Part of this work was presented at Lancefield 2008 International Symposium on Streptococci and Streptococcal Diseases, Porto Heli, Greece, June 22–26, 2008.

2Current affiliation: Clinic for Infectious Diseases, University Hospital Bern, Bern, Switzerland.

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