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Volume 15, Number 2—February 2009

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Natural Transmission of Zoonotic Babesia spp. by Ixodes ricinus Ticks

Claire A.M. Becker, Agnès Bouju-Albert, Maggy Jouglin, Alain Chauvin, and Laurence MalandrinComments to Author 
Author affiliations: Institut National de la Recherche Agronomique, Nantes, France (C.A.M. Becker, A. Bouju-Albert, M. Jouglin, A. Chauvin, L. Malandrin); Ecole Nationale Vétérinaire de Nantes, Nantes (C.A.M. Becker, A. Bouju-Albert, M. Jouglin, A. Chauvin, L. Malandrin)

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Figure

Microscopic appearance of Babesia sp. EU1 sporozoites isolated from tick salivary glands and of subsequent asexual development in erythrocytes. Sporozoites were stained with Giemsa and observed in the suspension of crushed salivary glands (A, B) and from salivary glands directly crushed between slides (C, D, E). Arrows indicate sporozoite dividing forms. A composite panel of asexual stages cultivated in sheep erythrocytes from these sporozoites is presented (F); developmental stages are indicate

Figure. Microscopic appearance of Babesia sp. EU1 sporozoites isolated from tick salivary glands and of subsequent asexual development in erythrocytes. Sporozoites were stained with Giemsa and observed in the suspension of crushed salivary glands (A, B) and from salivary glands directly crushed between slides (C, D, E). Arrows indicate sporozoite dividing forms. A composite panel of asexual stages cultivated in sheep erythrocytes from these sporozoites is presented (F); developmental stages are indicated by letters (D, dividing stages; M, free merozoites; S, schizont-like form; T, trophozoite). Scale bars = 5 μm.

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