Detection of Newly Described Astrovirus MLB1 in Stool Samples from Children
Figure 1. Astrovirus open reading frame (ORF) 1b alignments for design of pan-astrovirus primers. Astrovirus RNA polymerase sequences (ORF1b) were aligned at the amino acid level to define the conserved regions used for the design of primers SF0073 (A) and SF0076 (B). The numbers to the right of the sequences indicate the position of the last amino acid within each ORF1b sequence. Red boxes represent the specific regions that were reverse translated into the corresponding nucleic acid sequences used for the design of SF0073 (C) and SF0076 (D). Red sequences shown in the nucleotide alignments are the actual primer sequences.