Spotted Fever Group Rickettsia sp. Closely Related to R. japonica, Thailand
Figure. Phylogenetic analysis based on ompA gene (A) and rickettsial genus–specific 17-kDa antigen gene (B). Sequences were aligned by using the ClustalW software package (http://clustalw.ddbj.nig.ac.jp/top-j.html), and neighbor-joining phylogenetic tree construction and bootstrap analysis were conducted according to the Kimura 2-parameter method (www.ddbj.nig.ac.jp). Pairwise alignments were performed with an open-gap penalty of 10, a gap extension penalty of 0.5, and a gap distance of 8. Multiple alignments were also performed with the same values, and the phylogenetic branches were supported by bootstrap analysis with 1,000 replications (>800 were indicated). Rickettsia felis (CP000053) and R. canadensis (CP000409) were used as outgroups for ompA and 17-kDa antigen gene, respectively. The phylogenetic tree was constructed by using TreeView software version 1.5 (http://taxonomy.zoology.gla.ac.uk/rod/treeview.html). Scale bars indicate nucleotide substitutions (%) per site.