Volume 15, Number 4—April 2009
Concurrent Chikungunya and Dengue Virus Infections during Simultaneous Outbreaks, Gabon, 2007
|Towns||No. patients tested||No. CHIKV+||No. DENV-2+||No. CHIKV+/DENV-2+|
*CHIKV, chikungunya virus; DENV-2, dengue-2 virus; +, positive.
†RNA was extracted from 50 µL of plasma by using the ABI Prism 6100 Nucleic Acid PrepStation according to the manufacturer’s recommended procedures (Applied Biosystems, Foster City, CA, USA). Fifty-microliter aliquots of extracted RNA were then used in 100-µL High Capacity cDNA synthesis reactions according to the manufacturer’s instructions (Applied Biosystems). Finally, 10 µL of each cDNA reaction was then used as template for 50-µL quantitative PCRs that contained 200 nmol/L of probe and 900 nmol/L of each primer. The quantitative PCRs were then thermo-cycled in a 7500 Real-Time PCR system (Applied Biosystems) according to manufacturer’s recommended procedures. The probe used for the CHIKV, DENV, and DENV-2 assays were FAM-labeled with TAMRA quencher (Applied Biosystems).
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