Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

Volume 15, Number 4—April 2009

Dispatch

Concurrent Chikungunya and Dengue Virus Infections during Simultaneous Outbreaks, Gabon, 2007

Eric M. LeroyComments to Author , Dieudoné Nkoghe, Benjamin Ollomo, Chimène Nze-Nkogue, Pierre Becquart, Gilda Grard, Xavier Pourrut, Rémi Charrel, Grégory Moureau, Angélique Ndjoyi-Mbiguino, and Xavier de Lamballerie
Author affiliations: Centre International de Recherches Médicales de Franceville, Franceville, Gabon (E.M. Leroy, D. Nkoghe, B. Ollomo, C. Nze-Nkogue, P. Becquart, G. Grard, X. Pourrut); Université de la Méditerranée, Marseille, France (R. Charrel, G. Moureau, X. De Lamballerie); Université des Sciences de la Santé, Libreville, Gabon (A. Ndjoyi-Mbiguino)

Main Article

Table

Positive test results for CHIKV and DENV-2 among febrile patients, by town, Gabon, 2007*†

Towns No. patients tested No. CHIKV+ No. DENV-2+ No. CHIKV+/DENV-2+
Libreville 686 249 45 6
Ntoum 3 1 0 0
Kango 7 3 0 0
Mitzic 6 4 0 0
Oyem 45 15 2 1
Minvoul 7 3 1 1
Cocobeach 19 0 6 0
Total 773 275 54 8

*CHIKV, chikungunya virus; DENV-2, dengue-2 virus; +, positive.
†RNA was extracted from 50 µL of plasma by using the ABI Prism 6100 Nucleic Acid PrepStation according to the manufacturer’s recommended procedures (Applied Biosystems, Foster City, CA, USA). Fifty-microliter aliquots of extracted RNA were then used in 100-µL High Capacity cDNA synthesis reactions according to the manufacturer’s instructions (Applied Biosystems). Finally, 10 µL of each cDNA reaction was then used as template for 50-µL quantitative PCRs that contained 200 nmol/L of probe and 900 nmol/L of each primer. The quantitative PCRs were then thermo-cycled in a 7500 Real-Time PCR system (Applied Biosystems) according to manufacturer’s recommended procedures. The probe used for the CHIKV, DENV, and DENV-2 assays were FAM-labeled with TAMRA quencher (Applied Biosystems).

Main Article

TOP