Figure 2. A) Phylogenetic tree (unweighted pair-grouping method with arithmetic means) constructed on the complete or near-complete nucleotide sequences of hepatitis E virus (HEV) genotype 3 isolates. Clustering of nucleotide sequences of 8 human patients infected with JIO strain of HEV and 5 swine infected with swJ19 strain of HEV is boxed by a solid line. Another clustering of local genotype 3 isolates from Hyogo Prefecture, Japan, is boxed by a dotted line. B) Phylogenetic tree (unweighted pair-grouping method with arithmetic means) constructed on a partial sequence of 412 nt in open reading frame (ORF) 2 (nt 5994-6405 of the US2 genome) of HEV genotype 3. Partial nucleotide sequences of 8 human JIO and 11 swine HEV swJI9 isolates (accession nos. AB094279–AB094289) are shown (shading). Analyses of full sequences of 5 of these 11 swine isolates were performed (swJ19-1, swJ19-2, swJ19-5, swJ19-7, and swJ19-8). Scale bars indicate nucleotide substitutions per site; boldface indicates isolates from humans.