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Volume 15, Number 6—June 2009

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Diagnosis of Queensland Tick Typhus and African Tick Bite Fever by PCR of Lesion Swabs

Jin-Mei Wang, Bernard J. HudsonComments to Author , Matthew R. Watts, Tom Karagiannis, Noel J. Fisher, Catherine Anderson, and Paul Roffey
Author affiliations: Royal North Shore Hospital, St. Leonards, Sydney, Queensland, Australia (J.-M. Wang, B.J. Hudson, M.R. Watts, T. Karagiannis, N.J. Fisher); Woodland Street Medical Practice, Balgowlah, New South Wales, Australia (C. Anderson); Charles Sturt University, Wagga Wagga, New South Wales, Australia (P. Roffey)

Main Article

Table

PCR results by specimen, relation to clinical signs, and doses of doxycycline taken, Australia*

Case no. Specimen type Duration of lesion, d Duration of fever, d No. 100-mg doxycycline doses PCR result
1
EDTA Blood, EDTA NA 3 2
Eschar Eschar, Amies, dry 6 3 2
Eschar Eschar, virus, dry 6 3 2 +
Vesicle Vesicle, virus, dry 6 3 2 +
Vesicle
Vesicle, virus, dry
7
4
4

2
Blood Blood, EDTA NA 3 0
Eschar Eschar, Amies, dry 6 3 0 +
Eschar Eschar, virus, dry 8 5 0 +
Eschar Eschar, virus, saline 10 7 4 +
Vesicle Vesicle, Amies, dry 1 3 0
Vesicle Vesicle, virus, dry 3 5 0
Vesicle Vesicle, virus, saline 5 7 4
Palatal lesion
Lesion, virus, dry
5
7
4
+
3
Blood Blood, EDTA NA 1 0
Eschar Eschar, virus, saline 10 2 1 +
Vesicle
Vesicle, virus, saline
6
2
1
+
4
Blood Blood, EDTA NA 5 2
Eschar Eschar, virus, dry 10 5 2 +
Vesicle Vesicle, virus, dry 4 5 2 +
Vesicle Vesicle, virus, dry 4 5 2 +

*NA, not applicable.

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