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Volume 15, Number 6—June 2009

Dispatch

Murine Typhus in Child, Yucatan, Mexico

Jorge E. Zavala-CastroComments to Author , Jorge E. Zavala-Velázquez, and Justo Eduardo Sulú Uicab
Author affiliations: Universidad Autónoma de Yucatán, Mérida Yucatán, Mexico

Main Article

Table

Methods used to diagnose human Rickettsia typhi infection, Yucatan, Mexico, 2007*

Sample PCR, % identity
IFA, titer†
Citrate synthase (gltA) (382 bp)‡ 17-kDa gene (434 bp)§ IgM IgG
Rickettsia typhi Wilmington strain 100 (AE017197.1) 100 (M28481.1), 99 (AE017197.1) 256 128
R. felis URRWXCal2 NR 89 (CP000053.1) ND
R. rickettsii Sheila Smith 92 (CP000848.1) 89 (CP000848.1) Neg 128
R. akari Hartford strain 91 (CP000847.1) 96 (CP000847.1) Neg 64

*IFA, indirect immunofluorescence assay; Ig, immunoglobulin; NR, not represented in the first 100 sequences with a significant alignment using BLAST (9); ND, not determined; Neg, negative.
†Titers refer to the reciprocal of the dilution.
‡Primers: RpCS.1258 5′-attgcaaaaa gtaccgtaaaca-3′ and RpCS.877 5′-gccccgccgtgggcaggccccc-3′.
§Primers: 17-kDa 5′-gctcttgcaacttctatgtt-3′ and 5′-cattgttcgtcaggttggcg-3′.

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